Automated, semi‐automated, and manual analyses of anti‐cardiolipin and anti‐β2‐glycoprotein I antibodies in women with a history of miscarriage. Issue 2 (4th October 2012)

• Record Type: Journal Article
• Authors: Gutensohn, K.; Vossen, D.; Strate, A.; Kersten, J. F.; Hofbauer, M.; Krieger, T.
• Is Part Of: International journal of laboratory hematology. Volume 35:Issue 2(2013:Apr.)
• Page Start: 150
• Page End: 162
• Publication Date: 2012-10-04
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.12011
• Languages: English
• ISSNs: 1751-5521

Abstract

<abstract abstract-type="main" xml:lang="en" id="ijlh12011-abs-0001"> <title>Summary</title> <sec id="ijlh12011-sec-0001" sec-type="section"> <title>Introduction</title> <p>Anti‐cardiolipin and β2‐glycoprotein I antibodies represent important diagnostic parameters in routine hematology. In this study, five different automated, semi‐automated, and manual immunoassays detecting IgG/IgM anti‐cardiolipin and anti‐β<sub>2</sub>‐glycoprotein I antibodies were tested.</p> </sec> <sec id="ijlh12011-sec-0002" sec-type="section"> <title>Methods</title> <p>A total of 162 samples from women with a history of miscarriage were recruited from 110 different G&O outpatient centers in Germany.</p> </sec> <sec id="ijlh12011-sec-0003" sec-type="section"> <title>Results</title> <p>For both anti‐cardiolipin and anti‐β<sub>2</sub>‐glycoprotein I antibodies, considerable differences in the percentage of positive results were seen between all five methods, and itemization of all positive test results revealed a poor accordance. These findings were confirmed by Cohen's kappa coefficients.</p> </sec> <sec id="ijlh12011-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our study revealed a moderate to poor accordance between five different test systems for anti‐cardiolipin and anti‐β<sub>2</sub>‐glycoprotein I antibodies. Such deviations may result in clinical misinterpretation of data and may lead to wrong therapeutic consequences. Therefore, further standardization of all tests for anti‐phospholipid antibodies should be achieved.</p> </sec> </abstract>