Angiotensin‐Converting Enzyme Inhibitory Activity and Antioxidant Properties of Nepeta crassifolia Boiss & Buhse and Nepeta binaludensis Jamzad. (13th June 2012)

• Record Type: Journal Article
• Authors: Tundis, Rosa; Nadjafi, Farsad; Menichini, Francesco
• Is Part Of: Phytotherapy research. Volume 27:Number 4(2013:Apr.)
• Page Start: 572
• Page End: 580
• Publication Date: 2012-06-13
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.4757
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This article reports phytochemical and biological studies on <italic>Nepeta binaludensis</italic> and <italic>Nepeta crassifolia</italic>. Both species were investigated for their angiotensin‐converting enzyme (ACE) inhibitory activity and antioxidant properties through three <italic>in vitro</italic> models [2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2, 2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assay]. Aerial parts were extracted with methanol and partitioned between water and subsequently <italic>n</italic>‐hexane, ethyl acetate and <italic>n</italic>‐butanol. <italic>N. binaludensis</italic> methanol extract exerted significantly higher reducing power (1.9 μM Fe(II)/g) than did the positive control butylhydroxytoluene (63.2 μM Fe(II)/g) in FRAP assay. The highest DPPH radical scavenging activity was found for <italic>N. crassifolia</italic>, with IC<sub>50</sub> values of 9.6 and 12.1 µg/mL for ethyl acetate and <italic>n</italic>‐butanol fractions, respectively. <italic>n</italic>‐Butanol fraction of both species showed the highest ACE inhibitory activity, with IC<sub>50</sub> values of 59.3 and 81.7 µg/mL for <italic>N. binaludensis</italic> and <italic>N. crassifolia</italic>, respectively. Phytochemical investigations resulted in the isolation of ursolic acid, oleanolic acid, apigenin, luteolin and ixoroside. Apigenin‐7‐<italic>O</italic>‐glucoside, 8‐hydroxycirsimaritin and cirsimaritin were furthermore identified in <italic>N. crassifolia</italic> ethyl acetate‐soluble fraction. Nepetanudoside B was isolated from the <italic>n</italic>‐butanol fraction of <italic>N. binaludensis</italic>. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>