Ammonia‐oxidizing archaea and nitrite‐oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system. Issue 1 (9th August 2012)

• Record Type: Journal Article
• Authors: Brown, Monisha N.; Briones, Aurelio; Diana, James; Raskin, Lutgarde
• Is Part Of: FEMS microbiology ecology. Volume 83:Issue 1(2013)
• Page Start: 17
• Page End: 25
• Publication Date: 2012-08-09
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/j.1574-6941.2012.01448.x
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem1448-abs-0001"> <title>Abstract</title> <p>This study analysed the nitrifier community in the biofilter of a zero discharge, recirculating aquaculture system (RAS) for the production of marine shrimp in a low density (low ammonium production) system. The ammonia‐oxidizing populations were examined by targeting 16S rRNA and <italic>amoA</italic> genes of ammonia‐oxidizing bacteria (AOB) and archaea (AOA). The nitrite‐oxidizing bacteria (NOB) were investigated by targeting the 16S rRNA gene. Archaeal <italic>amoA</italic> genes were more abundant in all compartments of the RAS than bacterial <italic>amoA</italic> genes. Analysis of bacterial and archaeal <italic>amoA</italic> gene sequences revealed that most ammonia oxidizers were related to <italic>Nitrosomonas marina</italic> and <italic>Nitrosopumilus maritimus</italic>. The NOB detected were related to <italic>Nitrospira marina</italic> and <italic>Nitrospira moscoviensis, </italic> and <italic>Nitrospira marina</italic>‐type NOB were more abundant than <italic>N. moscoviensis</italic>‐type NOB. Water quality and biofilm attachment media played a role in the competitiveness of AOA over AOB and <italic>Nitrospira marina‐</italic>over <italic>N. moscoviensis‐</italic>type NOB.</p> </abstract>