Comparative evaluation of different calcium phosphate‐based bone graft granules – an in vitro study with osteoblast‐like cells. Issue 4 (24th October 2011)

• Record Type: Journal Article
• Authors: Bernhardt, Anne; Lode, Anja; Peters, Fabian; Gelinsky, Michael
• Is Part Of: Clinical oral implants research. Volume 24:Issue 4(2013:Apr.)
• Page Start: 441
• Page End: 449
• Publication Date: 2011-10-24
• Subjects: Dental implants -- Research -- Periodicals; 617.69
• DOI: 10.1111/j.1600-0501.2011.02350.x
• Languages: English
• ISSNs: 0905-7161

Abstract

<abstract abstract-type="main" id="clr2350-abs-0001"> <title>Abstract</title> <sec id="clr2350-sec-0001" sec-type="section"> <title>Objective</title> <p>Granule‐shaped calcium phosphate‐based bone graft materials are often required for bone regeneration especially in implant dentistry. Two newly developed bone graft materials are Ceracell<sup>®</sup>, an open‐celled highly porous bioceramic from β‐tricalcium phosphate (β‐TCP) under addition of bioglass and Osseolive<sup>®</sup>, an open porous glass ceramic with the general formula Ca<sub>2</sub>KNa(PO<sub>4</sub>)<sub>2</sub>. The goal of this study was to characterize different modifications of the two bone graft materials <italic>in vitro</italic> in comparison to already established ceramic bone grafts Cerasorb M<sup>®</sup>, NanoBone<sup>®</sup> and BONIT Matrix<sup>®</sup>.</p> </sec> <sec id="clr2350-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Adhesion and proliferation of SaOS‐2 osteoblast‐like cells were evaluated quantitatively by determining DNA content and lactate dehydrogenase (LDH) activity and qualitatively by scanning electron microscopy (SEM). In addition, MTT cell‐vitality staining was applied to confirm the attachment of viable cells to the different materials. Osteogenic differentiation was evaluated by measurement of alkaline phosphatase (ALP) activity as well as gene expression analysis of osteogenic markers using reverse transcriptase PCR.</p> </sec> <sec id="clr2350-sec-0003" sec-type="section"> <title>Results</title> <p>DNA content and LDH activity revealed good cell attachment and proliferation for Ceracell and Cerasorb M. When pre‐incubated with cell‐culture medium, also Osseolive showed good cell attachment and proliferation. Attachment and proliferation of osteoblast‐like cells on NanoBone and BONIT Matrix was very low, even after pre‐incubation with cell‐culture medium. Specific ALP activity on Ceracell<sup>®</sup>, Osseolive <sup>®</sup> and Cerasorb M<sup>®</sup> increased with time and expression of bone‐related genes ALP, osteonectin, osteopontin and bone sialoprotein II was demonstrated.</p> </sec> <sec id="clr2350-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Ceracell as well as Osseolive granules support proliferation and osteogenic differentiation <italic>in vitro</italic> and may be promising candidates for <italic>in vivo</italic> applications.</p> </sec> </abstract>