11α hydroxylation of 16α, 17‐epoxyprogesterone in biphasic ionic liquid/water system by Aspergillus ochraceus. Issue 2 (16th May 2012)

• Record Type: Journal Article
• Authors: Mao, Shuhong; Hua, Boyuan; Wang, Na; HU, Xiaojie; Ge, Zhijiang; Li, Yanqing; Liu, Sha; Lu, Fuping
• Is Part Of: Journal of chemical technology & biotechnology. Volume 88:Issue 2(2013:Feb.)
• Page Start: 287
• Page End: 292
• Publication Date: 2012-05-16
• Subjects: Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.3828
• Languages: English
• ISSNs: 0268-2575

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p> <bold>BACKGROUND: The improved efficiency of steroid biotransformation using the biphasic system is generally attributed to the positive effect on the solubility of substrate in aqueous media. A promising alternative for the application of organic solvents in biphasic systems is the use of ionic liquids (ILs). This study aims to investigate the applicability of the biphasic ILs/water system for 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by <italic>Aspergillus ochraceus</italic>.</bold> </p> <p> <bold>RESULTS: Of the seven ILs tested, [C<sub>3</sub>mim][PF<sub>6</sub>] exhibited the best biocompatibility, with markedly improved biotransformation efficiency. In the [C<sub>3</sub>mim][PF<sub>6</sub>]‐based biphasic system, substrate conversion reached 90% under the condition in which buffer pH, volume ratio of buffer to ILs, cell concentration, and substrate concentration were 4.8, 10/1, 165 g L<sup>−1</sup> and 20 g L<sup>−1</sup>, respectively. This is more efficient than that of the monophasic aqueous system. The effects of the cations and anions of these ILs on the 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by <italic>A. ochraceus</italic> is also discussed.</bold> </p> <p> <bold>CONCLUSION: The above results showed that IL/water biphasic system improved the efficiency of 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by <italic>A. ochraceus, </italic> thus suggesting the potential industrial application of ILs‐based biphasic systems for steroid biotransformation. © 2012 Society of Chemical Industry</bold> </p> </abstract>