BkaR is a TetR‐type repressor that controls an operon associated with branched‐chain keto‐acid metabolism in Mycobacteria. Issue 2 (8th July 2013)

• Record Type: Journal Article
• Authors: Balhana, Ricardo J.C.; Swanston, Sade N.; Coade, Stephen; Withers, Mike; Sikder, Mahmudul Hasan; Stoker, Neil G.; Kendall, Sharon L.
• Is Part Of: FEMS microbiology letters. Volume 345:Issue 2(2013:Aug.)
• Page Start: 132
• Page End: 140
• Publication Date: 2013-07-08
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12196
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12196-abs-0001"> <title>Abstract</title> <p>This study describes how <italic>bkaR</italic>, a highly conserved mycobacterial TetR‐like transcriptional repressor, regulates a number of nearby genes that have associations with branched‐chain keto‐acid metabolism. <italic>bkaR</italic> (<italic>MSMEG_4718</italic>) was deleted from the nonpathogenic species <italic>Mycobacterium smegmatis</italic>, and changes in global gene expression were assessed using microarray analysis and reporter gene studies. <italic>bkaR</italic> was found to directly control the expression of 10 genes in <italic>M. smegmatis</italic>, and its ortholog in <italic>Mycobacterium tuberculosis</italic> (<italic>Rv2506</italic>) is predicted to control at least 12 genes. A conserved operator motif was identified, and binding of purified recombinant <italic>M. tuberculosis </italic>BkaR to the motif was demonstrated. Analysis of the stoichiometry of binding showed that BkaR binds to the motif as a dimer.</p> </abstract>