Interleukin‐6‐induced satellite cell proliferation is regulated by induction of the JAK2/STAT3 signalling pathway through cyclin D1 targeting. (21st July 2013)

• Record Type: Journal Article
• Authors: Kurosaka, M.; Machida, S.
• Is Part Of: Cell proliferation. Volume 46:Number 4(2013:Aug.)
• Page Start: 365
• Page End: 373
• Publication Date: 2013-07-21
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12045
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12045-abs-0001"> <title>Abstract</title> <sec id="cpr12045-sec-0001" sec-type="section"> <title>Objectives</title> <p>To determine whether interleukin‐6 (IL‐6) stimulates rat muscle satellite cell proliferation in culture, and if so, to clarify the signalling mechanisms.</p> </sec> <sec id="cpr12045-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Primary satellite cells were isolated from thirty male F344 rats, 11 weeks of age. IL‐6 at concentrations of 0.01, 0.1, 1, 10 or 100 ng/ml was added to culture media.</p> </sec> <sec id="cpr12045-sec-0003" sec-type="section"> <title>Results</title> <p>IL‐6 at 0.01–1 ng/ml induced dose‐dependent increase in cell proliferation. After treatment with 1 ng/ml IL‐6, cell proliferation increased by 31%, and p‐STAT3<sup>+</sup>/MyoD<sup>+</sup> cells increased in number compared to those in control media (<italic>P</italic> < 0.05). Inhibitors of JAK2 (AG 490) and STAT3 (STAT3 peptide) blocked the increase in BrdUrd<sup>+</sup> cell numbers at 6 h post stimulation with 1 ng/ml IL‐6 (<italic>P</italic> < 0.05). Furthermore, cyclin D1 mRNA expression and cyclin D1<sup>+</sup>/MyoD<sup>+</sup> cell numbers significantly increased in cultures treated with 1 ng/ml IL‐6 compared to those in control media (<italic>P</italic> < 0.05). In contrast, treatment with 10 and 100 ng/ml IL‐6 did not stimulate cell proliferation. Treatment with 10 ng/ml IL‐6 induced greater SOCS3 mRNA expression than with 1 ng/ml IL‐6 and control media. Moreover, co‐localization of SOCS3 and myogenin was observed after treatment with 10 ng/ml IL‐6.</p> </sec> <sec id="cpr12045-sec-0004" sec-type="section"> <title>Conclusions</title> <p>IL‐6 induced dose‐dependent increase in satellite cell proliferation by activating the JAK2/STAT3/cyclin D1 pathway.</p> </sec> </abstract>