Isolation and characterization of Keratinibaculum paraultunense gen. nov., sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. Issue 1 (27th June 2013)

• Record Type: Journal Article
• Authors: Huang, Yan; Sun, Yingjie; Ma, Shichun; Chen, Lu; Zhang, Hui; Deng, Yu
• Is Part Of: FEMS microbiology letters. Volume 345:Issue 1(2013:Aug.)
• Page Start: 56
• Page End: 63
• Publication Date: 2013-06-27
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12184
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12184-abs-0001"> <title>Abstract</title> <p>A novel thermophilic, anaerobic, keratinolytic bacterium designated KD‐1 was isolated from grassy marshland. Strain KD‐1 was a spore‐forming rod with a Gram‐positive type cell wall, but stained Gram‐negative. The temperature, pH, and NaCl concentration range necessary for growth was 30–65 °C (optimum 55 °C), 6.0–10.5 (optimum 8.0–8.5), and 0–6% (optimum 0.2%) (w/v), respectively. Strain KD‐1 possessed extracellular keratinase, and the optimum activity of the crude enzyme was pH 8.5 and 70 °C. The enzyme was identified as a thermostable serine‐type protease. The strain was sensitive to rifampin, chloramphenicol, kanamycin, and tetracycline and was resistant to erythromycin, neomycin, penicillin, and streptomycin. The main cellular fatty acid was predominantly C15:0 iso (64%), and the G+C content was 28 mol%. Morphological and physiological characterization, together with phylogenetic analysis based on 16S rRNA gene sequencing identified KD‐1 as a new species of a novel genus of <italic>Clostridiaceae</italic> with 95.3%, 93.8% 16S rRNA gene sequence similarity to <italic>Clostridium ultunense</italic> BS<sup>T</sup> (DSM 10521<sup>T</sup>) and <italic>Tepidimicrobium xylanilyticum</italic> PML14<sup>T</sup> (= JCM 15035<sup>T</sup>), respectively. We propose the name <italic>Keratinibaculum paraultunense</italic> gen. nov., sp. nov., with KD‐1 (=JCM 18769<sup>T</sup> =DSM 26752<sup>T</sup>) as the type strain.</p> </abstract>