Acquisition of t(11;14) in a patient with chronic lymphocytic leukemia carrying both t(14;19)(q32;q13.1) and +12. (31st May 2013)

• Record Type: Journal Article
• Authors: Nishida, Yuhki; Takeuchi, Kengo; Tsuda, Kenji; Ugai, Tomotaka; Sugihara, Hiroki; Yamakura, Masayuki; Takeuchi, Masami; Matsue, Kosei
• Is Part Of: European journal of haematology. Volume 91:Number 2(2013:Aug.)
• Page Start: 179
• Page End: 182
• Publication Date: 2013-05-31
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Blood -- Periodicals; 616.15005
• DOI: 10.1111/ejh.12119
• Languages: English
• ISSNs: 0902-4441

Abstract

<abstract abstract-type="main" id="ejh12119-abs-0001"> <title>Abstract</title> <p>A rare recurrent chromosomal translocation, t(14;19)(q32;q13), has been identified in a variety of B‐cell malignancies, including chronic lymphocytic leukemia (CLL). We report a unique case of CLL in a patient carrying both trisomy 12 and t(14;19) (q32;q13.1), in whom t(11;14)(q13;q32) developed at relapse. The patient was a 77‐yr‐old woman, and her lymphoma cells at presentation showed CD5<sup>+</sup>, CD10<sup>−</sup>, CD19<sup>+</sup>, CD20<sup>+</sup>(dim), CD23<sup>+</sup>, CD38<sup>+</sup>, and CD11c<sup>+</sup>. At relapse, the patient's lymphoma cells showed positive staining for cyclin D1 in addition to CD5, CD20, and CD23. Lymphoma cells in specimens at both presentation and relapse were positive for lymphoid enhancer factor 1 (LEF1) and negative for sex‐determining region Y‐box 11 (SOX11). <italic>IGH‐BCL1 </italic>FISH became positive at relapse. Split FISH assay using <italic>BCL1</italic>, <italic> BCL3</italic>, <italic> IGH</italic>, and <italic>CCND1</italic> probes on lymph node specimens obtained at presentation and at autopsy confirmed that the translocation of <italic>BCL3</italic> was solely detected in the lymph node at presentation and detected <italic>BCL3</italic> and <italic>CCND1</italic> translocations in the specimen at autopsy. These observations indicated that <italic>IGH‐BCL3</italic> and <italic>IGH‐CCND1</italic> had occurred in the same clone after treatment of the disease. In line with immunohistochemical and cytogenetic studies, additional PCR analysis of the FR3‐JH region showed the same sequence derived from IGHV4‐34 in specimens obtained at disease onset and relapse.</p> </abstract>