Inhibitory effect of Pseudomonas putida nitrogen‐related phosphotransferase system on conjugative transfer of IncP‐9 plasmid from Escherichia coli. Issue 2 (19th June 2013)

• Record Type: Journal Article
• Authors: Inoue, Kei; Miyazaki, Ryo; Ohtsubo, Yoshiyuki; Nagata, Yuji; Tsuda, Masataka
• Is Part Of: FEMS microbiology letters. Volume 345:Issue 2(2013:Aug.)
• Page Start: 102
• Page End: 109
• Publication Date: 2013-06-19
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12188
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" id="fml12188-abs-0001"> <title>Abstract</title> <p>Conjugative plasmid transfer systems have been well studied, but very little is known about the recipient factors that control horizontal transmission. A self‐transmissible IncP‐9 naphthalene catabolic plasmid, NAH7, carries the <italic>traF</italic> gene, whose product is considered to be a host‐range modifier of NAH7, because its <italic>traF</italic> deletion mutant (NAH7dF) is transmissible from <italic>Pseudomonas putida</italic> to <italic>P. putida</italic> and <italic>Escherichia coli</italic> and from <italic>E. coli</italic> to <italic>E. coli</italic>, but not from <italic>E. coli</italic> to <italic>P. putida</italic>. In this study, transposon mutagenesis of <italic>P. putida</italic> KT2440 was performed to isolate the mutants that could receive NAH7dF from <italic>E. coli</italic>. The mutants had the transposon insertions in <italic>ptsP</italic> or <italic>ptsO</italic>, encoding two of three components of the nitrogen‐related phosphotransferase system (PTS<sup>Ntr</sup>). The KT2440 derivative lacking <italic>ptsN</italic>, encoding the remaining component of PTS<sup>Ntr</sup>, was also able to receive NAH7dF. These results indicated that the PTS<sup>Ntr</sup> in <italic>P. putida</italic> is involved in inhibition of conjugative transfer of NAH7dF from <italic>E. coli</italic>. Our further experiments using site‐directed mutants suggested the indirect involvement of the phosphorylated form of PtsO in the inhibition of the conjugative transfer. Conjugative transfer of NAH7 and another IncP‐9 plasmid, pWW0, from <italic>E. coli</italic> was partially inhibited by the PtsO function in KT2440.</p> </abstract>