In Vitro Activity of Xanthorrhizol Isolated from the Rhizome of Javanese Turmeric (Curcuma xanthorrhiza Roxb.) Against Candida albicans Biofilms. (12th September 2012)

• Record Type: Journal Article
• Authors: Rukayadi, Yaya; Hwang, Jae‐Kwan
• Is Part Of: Phytotherapy research. Volume 27:Number 7(2013:Jul.)
• Page Start: 1061
• Page End: 1066
• Publication Date: 2012-09-12
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.4834
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The purpose of this study was to investigate the activity of xanthorrhizol isolated from <italic>Curcuma xanthorrhiza</italic> Roxb. on <italic>Candida albicans</italic> biofilms at adherent, intermediate, and mature phase of growth. <italic>C. albicans</italic> biofilms were formed in flat‐bottom 96‐well microtiter plates. The biofilms of <italic>C. albicans</italic> at different phases of development were exposed to xanthorrhizol at different concentrations (0.5 µg/mL ‐ 256 µg/mL) for 24 h. The metabolic activity of cells within the biofilms was quantified using the XTT reduction assay. Sessile minimum inhibitory concentrations (SMICs) were determined at 50% and 80% reduction in the biofilm OD<sub>490</sub> compared to the control wells. The SMIC<sub>50</sub> and SMIC<sub>80</sub> of xanthorrhizol against 18 <italic>C. albicans</italic> biofilms were 4 – 16 µg/mL and 8 – 32 µg/mL, respectively. The results demonstrated that the activity of xanthorrhizol in reducing <italic>C. albicans</italic> biofilms OD<sub>490</sub> was dependent on the concentration and the phase of growth of biofilm. Xanthorrhizol at concentration of 8 µg/mL completely reduced in biofilm referring to XTT‐colorimetric readings at adherent phase, whereas 32 µg/mL of xanthorrhizol reduced 87.95% and 67.48 % of biofilm referring to XTT‐colorimetric readings at intermediate and mature phases, respectively. Xanthorrhizol displayed potent activity against <italic>C. albicans</italic> biofilms <italic>in vitro</italic> and therefore might have potential therapeutic implication for biofilm‐associated candidal infections. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>