Complete sequence of Erwinia piriflorinigrans plasmids pEPIR37 and pEPIR5 and role of pEPIR37 in pathogen virulence. (29th October 2012)

• Record Type: Journal Article
• Authors: Barbé, S.; Llop, P.; Blom, J.; Cabrefiga, J.; Goesmann, A.; Duffy, B.; Montesinos, E.; Smits, T. H. M.; López, M. M.
• Is Part Of: Plant pathology. Volume 62:Number 4(2013:Aug.)
• Page Start: 786
• Page End: 798
• Publication Date: 2012-10-29
• Subjects: Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.12002
• Languages: English
• ISSNs: 0032-0862

Abstract

<abstract abstract-type="main" id="ppa12002-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Erwinia piriflorinigrans</italic> is a newly described pathogen causing necrosis of pear blossoms. Complete sequencing of the 37‐kb plasmid pEPIR37 common to 27 <italic>E. piriflorinigrans</italic> strains revealed homology to sequences of the ubiquitous plasmids pEA29 of the fire blight pathogen <italic>E. amylovora</italic>, plasmid pEP36 of <italic>E. pyrifoliae</italic>, plasmid pEJ30 of <italic>Erwinia</italic> sp. from Japan, and genomic regions of the related Rosaceae epiphytic <italic>Erwinia</italic> species <italic>E. tasmaniensis</italic> and <italic>E. billingiae</italic>. A second 5·5‐kb cryptic plasmid pEPIR5, found in 12 <italic>E. piriflorinigrans</italic> strains, was also sequenced revealing mobilization and replication proteins with similarities to many small ColE1‐type plasmids in <italic>Erwinia</italic> spp. and other enterobacteria. Functional analyses of pEPIR37 introduced into a strain of <italic>E. amylovora</italic> cured of pEA29 plasmid, which has a reduced virulence, showed a role in increasing symptom development similar to that observed in <italic>E. amylovora</italic> carrying plasmid pEA29.</p> </abstract>