Mantle cell lymphoma cell lines show no evident immunoglobulin heavy chain stereotypy but frequent light chain stereotypy. Issue 8 (August 2013)

• Record Type: Journal Article
• Authors: Pighi, Chiara; Barbi, Stefano; Bertolaso, Anna; Zamò, Alberto
• Is Part Of: Leukemia & lymphoma. Volume 54:Issue 8(2013:Aug.)
• Page Start: 1747
• Page End: 1755
• Publication Date: 2013-08
• Subjects: Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2012.758843
• Languages: English
• ISSNs: 1042-8194

Abstract

<abstract> <title>Abstract</title> <p>Mantle cell lymphoma shows a peculiar immunogenetic profile, but the functional consequences of this fact are unknown. We have determined the precise sequences of rearranged heavy and light chain genes in several mantle cell lymphoma cell lines and investigated the presence of heavy and light chain stereotypy. These cell lines use <italic>IGHV</italic> and <italic>IGLV</italic> genes that are known to be preferentially rearranged in mantle cell lymphoma, but we found no evidence of heavy chain stereotypy. In contrast, one cell line (Mino) showed a nearly identical light chain complementarity-determining region 3 when compared to the only published light chain cluster. Two cell line couples (Jeko-1/UPN-2 and JVM-2/JVM-13) showed a highly similar light chain that satisfied the criteria for stereotypy. Our data show that mantle cell lymphoma cell lines resemble the <italic>IGHV</italic> and <italic>IGLV</italic> usage of mantle cell lymphoma, and foster the hypothesis that light chain stereotypy might be under-recognized.</p> </abstract>