Adaptation of Saccharomyces cerevisiae to toxic manganese concentration triggers changes in inorganic polyphosphates. Issue 5 (3rd June 2013)

• Record Type: Journal Article
• Authors: Andreeva, Nadezhda; Ryazanova, Lubov; Dmitriev, Vladimir; Kulakovskaya, Tatiana; Kulaev, Igor
• Is Part Of: FEMS yeast research. Volume 13:Issue 5(2013)
• Page Start: 463
• Page End: 470
• Publication Date: 2013-06-03
• Subjects: Yeast -- Periodicals; Yeasts -- Periodicals; 579.562
• DOI: 10.1111/1567-1364.12049
• Languages: English
• ISSNs: 1567-1356

Abstract

<abstract abstract-type="main" id="fyr12049-abs-0001"> <title>Abstract</title> <p>The ability of <italic>Saccharomyces cerevisiae</italic> to adapt to toxic Mn²⁺ concentration (4 mM) after an unusually long lag phase has been demonstrated for the first time. The mutants lacking exopolyphosphatase PPX1 did not change the adaptation time, whereas the mutants lacking exopolyphosphatase PPN1 reduced the lag period compared with the wild‐type strains. The cell populations of WT and <italic>ΔPPN1</italic> in the stationary phase at cultivation with Mn²⁺ contained a substantial number of enlarged cells with a giant vacuole. The adaptation correlated with the triggering of polyphosphate metabolism: the drastic increase in the rate and chain length of acid‐soluble polyphosphate. The share of this fraction, which is believed to be localized in the cytoplasm, increased to 76%. Its average chain length increased to 200 phosphate residues compared with 15 at the cultivation in the absence of manganese. DAPI‐stained inclusions in the cytoplasm were accumulated in the lag phase during the cultivation with Mn²⁺.</p> </abstract>