Alterations in transcriptome and proteome on metallothioneins following oxidative stress induced by sublethal doses of cadmium and gamma rays in Plantago ovata. (July 2013)

• Record Type: Journal Article
• Authors: Ghoshal, Nirmalya; Talapatra, Shonima; Moulick, Amitava; Chakraborty, Anindita; Raychaudhuri, Sarmistha Sen
• Is Part Of: International journal of radiation biology. Volume 89:Number 7(2013:Jul.)
• Page Start: 571
• Page End: 582
• Publication Date: 2013-07
• Subjects: Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2013.782109
• Languages: English
• ISSNs: 0955-3002

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl<sub>2</sub>) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in <italic>Plantago ovata</italic> Forsk (<italic>P. ovata</italic>).</p> <p> <italic>Materials and methods</italic>: Chlorophyll content was estimated to study the stress response in <italic>P. ovata</italic> seedlings following exposure to gamma irradiation and CdCl<sub>2</sub>. Lipid peroxidation and proline content, two oxidative stress markers, were also studied. The level of metallothionein gene and protein expression was further investigated using polymerase chain reaction (PCR), reverse transcription-PCR (RT-PCR), immunocytochemistry and flow cytometry.</p> <p> <italic>Results</italic>: Three MT genes of <italic>P. ovata</italic> namely <italic>PoMT 1, PoMT 2</italic> and <italic>PoMT 3</italic> were isolated, sequenced and characterized and their expressions were found to be altered in the case of both oxidative stresses in a dose-dependent and tissue-specific manner. The results were in agreement with the observations from immunocytochemistry and FACS analysis.</p> <p> <italic>Conclusions</italic>: The results suggest that both gamma irradiation and CdCl<sub>2</sub> alter redox balance in <italic>P. ovata</italic>. The metallothionein gene may play an important role in metal tolerance and stress balance. It is conjectured that the stress-mediated imbalance is maintained by altered MT gene and protein expression.</p> </abstract>