A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes. (July 2013)

• Record Type: Journal Article
• Authors: Poschmann, Gereon; Lendzian, Anna; Uszkoreit, Julian; Eisenacher, Martin; Borght, Ann Vander; Ramaekers, Frans C.; Meyer, Helmut Erich; Stühler, Kai
• Is Part Of: Archives of physiology and biochemistry. Volume 119:Number 3(2013)
• Page Start: 114
• Page End: 125
• Publication Date: 2013-07
• Subjects: Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.3109/13813455.2013.789529
• Languages: English
• ISSNs: 1381-3455

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Small cell lung cancers (SCLC) are heterogeneous and tumours differ in growth characteristics and treatment resistance. <italic>Objective</italic>: To get insight into the underlying protein profiles responsible for this heterogeneity, two subtypes of SCLC cells mutually differing in chemo resistance properties and growth characteristics are analysed. <italic>Materials and methods</italic>: Two different electrophoresis approaches in combination with mass spectrometry were used to detect differences between the SCLC cell lines GLC1 and GLC1M13: IEF/SDS-PAGE as well as cetyltrimethylammonium bromide (CTAB)-SDS-PAGE. <italic>Results</italic>: Altogether 60 non redundant differentially expressed proteins were found of which 5 were verified by Western Blot analysis. <italic>Discussion</italic>: Most of these proteins identified are involved in processes of tumour progression. Therefore, these proteins are interesting candidates for further functional analysis. <italic>Conclusion</italic>: Additional CTAB-SDS page is a complementary method to IEF-SDS page revealing a complete new subset of proteins differentially expressed between GLC1 and GLC1 M13 cells SCLC subtypes.</p> </abstract>