Expression profiles of aquaporin homologues and petal movement during petal development in Tulipagesneriana. Issue 3 (20th November 2012)

• Record Type: Journal Article
• Authors: Azad, Abul Kalam; Hanawa, Ryosuke; Ishikawa, Takahiro; Sawa, Yoshihiro; Shibata, Hitoshi
• Is Part Of: Physiologia plantarum. Volume 148:Issue 3(2013:Jul.)
• Page Start: 397
• Page End: 407
• Publication Date: 2012-11-20
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/j.1399-3054.2012.01717.x
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Previously, we have characterized two tonoplast intrinsic proteins (TIPs) and four plasma membrane intrinsic proteins (PIPs) from the 2‐day‐old petals of tulip (<italic>Tulipa gesneriana</italic>). In this study, we analyzed the development of tulip petals and stems, temperature‐dependent petal movement, the amount of <sup>3</sup>H<sub>2</sub>O transported into petals and stems during petal movement, and the transcript levels of two <italic>TIP</italic> (<italic>TgTIP1;1</italic> and <italic>TgTIP1;2</italic>) and four <italic>TgPIP</italic> genes in petals and stems, from the first day of petal opening to day 12. The development of the petals and stems was completed by days 6 and 9, respectively, after the first day of petal opening. Temperature‐dependent petal movement and the amount of <sup>3</sup>H<sub>2</sub>O that was transported into petals could be detected at significant levels up to day 6 with petal movement reaching a peak at day 3. Real‐time reverse transcription‐polymerase chain reaction analysis revealed that <italic>TgTIP1;1</italic> and <italic>TgTIP1;2</italic> were expressed ubiquitously in petals, stems, leaves, bulbs and roots. However, the expression level of <italic>TgTIP1;2</italic> was very low in bulbs. The expression of both <italic>TgTIP1</italic> genes was upregulated in close association with the development of petals but not with that of the stem. The four <italic>TgPIP</italic> genes were expressed at almost the same level during the development of the petals and the stem. However, the levels of the <italic>TgTIP1</italic> and <italic>TgPIP</italic> transcripts in petals decreased during the course of petal wilting from day 9 onwards. These results suggest that TgTIP1;1 and TgTIP1;2 may contribute to petal development.</p> </abstract>