Time‐dependent morphological alterations and viability of cultured human trabecular cells after exposure to Trypan blue. (10th December 2012)

• Record Type: Journal Article
• Authors: Tsaousis, Konstantinos T; Kopsachilis, Nikolaos; Tsinopoulos, Ioannis T; Dimitrakos, Stavros A; Kruse, Friedrich E; Welge‐Luessen, Ulrich
• Is Part Of: Clinical & experimental ophthalmology. Volume 41:Number 5(2013)
• Page Start: 484
• Page End: 490
• Publication Date: 2012-12-10
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1111/ceo.12018
• Languages: English
• ISSNs: 1442-6404

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="ceo12018-sec-0001" sec-type="section"> <title>Background</title> <p>To determine the time‐dependent toxicity of Trypan blue at 0.06% concentration in cultured human trabecular meshwork cells.</p> </sec> <sec id="ceo12018-sec-0002" sec-type="section"> <title>Methods</title> <p>Human trabecular meshwork cells cultured in vitro were exposed to Trypan blue and acute toxicity was evaluated. Cells were exposed for 5, 30, 60 s and for 5, 15, 30, 60 min to a commercially available Trypan blue preparation (Vision Blue; DORC International Zuidland, The Netherlands). Morphology was observed by phase‐contrast microscopy and cell viability was measured using Hoechst 33342 (Intergen; Purchase, NY, USA) and propidium iodide assays to determine the percentage of living and dead cells.</p> </sec> <sec id="ceo12018-sec-0003" sec-type="section"> <title>Results</title> <p>Morphological changes occurred mainly after 5 min of exposure to Trypan blue. Viability was 96.0% ± 3.6%, 94.8% ± 3.5% and 92.5% ± 4.4% after 5, 30 and 60 s of exposure, respectively; a significant toxic effect of Trypan blue was observed after 5, 15, 30 and 60 min of exposure with a viability of 85.0% ± 3.7%, 77.0% ± 6.7%, 70.8% ± 5.9% and 68.3% ± 8.7%, respectively.</p> </sec> <sec id="ceo12018-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Trypan blue is not toxic, in terms of cell viability, over an exposure time of up to 60 s; however, further exposure results in a gradual increase in damage of cultured human trabecular meshwork cells.</p> </sec> </abstract>