The Phytophthora parasitica RXLR effector Penetration‐Specific Effector 1 favours Arabidopsis thaliana infection by interfering with auxin physiology. Issue 2 (17th April 2013)

• Record Type: Journal Article
• Authors: Evangelisti, Edouard; Govetto, Benjamin; Minet‐Kebdani, Naïma; Kuhn, Marie‐Line; Attard, Agnès; Ponchet, Michel; Panabières, Franck; Gourgues, Mathieu
• Is Part Of: New phytologist. Volume 199:Issue 2(2013)
• Page Start: 476
• Page End: 489
• Publication Date: 2013-04-17
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12270
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12270-abs-0001"> <title>Summary</title> <p> <list id="nph12270-list-0001" list-type="bullet"> <list-item> <p>Pathogenic oomycetes have evolved RXLR effectors to thwart plant defense mechanisms and invade host tissues. We analysed the function of one of these effectors (Penetration‐Specific Effector 1 (PSE1)) whose transcript is transiently accumulated during penetration of host roots by the oomycete <italic>Phytophthora parasitica</italic>.</p> </list-item> <list-item> <p>Expression of PSE1 protein in tobacco (<italic>Nicotiana tabacum</italic> and <italic>Nicotiana benthamiana</italic>) leaves and in <italic>Arabidopsis thaliana</italic> plants was used to assess the role of this effector in plant physiology and in interactions with pathogens. A pharmacological approach and marker lines were used to charcterize the <italic>A. thaliana</italic> phenotypes.</p> </list-item> <list-item> <p>Expression of PSE1 in <italic>A. thaliana</italic> led to developmental perturbations associated with low concentrations of auxin at the root apex. This modification of auxin content was associated with an altered distribution of the PIN4 and PIN7 auxin efflux carriers. The PSE1 protein facilitated plant infection: it suppressed plant cell death activated by <italic>Pseudomonas syringae</italic> avirulence gene <italic>AvrPto</italic> and <italic>Phytophthora cryptogea</italic> elicitin cryptogein in tobacco and exacerbated disease symptoms upon inoculation of transgenic <italic>A. thaliana</italic> plantlets with <italic>P. parasitica</italic> in an auxin‐dependant manner.</p> </list-item> <list-item> <p>We propose that <italic>P. parasitica</italic> secretes the PSE1 protein during the penetration process to favour the infection by locally modulating the auxin content. These results support the hypothesis that effectors from plant pathogens may act on a limited set of targets, including hormones.</p> </list-item> </list> </p> </abstract>