RNase III‐dependent down‐regulation of ftsH by an artificial internal sense RNA in Anabaena sp. PCC 7120. Issue 2 (13th May 2013)

• Record Type: Journal Article
• Authors: Gao, Yuan; Gong, Yangmin; Xu, Xudong
• Is Part Of: FEMS microbiology letters. Volume 344:Issue 2(2013:Jul.)
• Page Start: 130
• Page End: 137
• Publication Date: 2013-05-13
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1111/1574-6968.12165
• Languages: English
• ISSNs: 0378-1097

Abstract

<abstract abstract-type="main" xml:lang="en" id="fml12165-abs-0001"> <title>Abstract</title> <sec id="fml12165-sec-0001" sec-type="section"> <p>RNase III is a group of dsRNA‐specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in <italic>Anabaena</italic> sp. PCC7120 are highly similar to RNase III enzymes. <italic>In vitro</italic>, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of <italic>ftsH</italic> (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (<italic>aaftsH</italic>) that was complementary to <italic>aftsH</italic>, an internal antisense RNA. The <italic>aaftsH</italic> interference was abolished by inactivation of <italic>alr0280</italic>, the RNase III‐encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to <italic>hen1</italic>, an RNA methyltransferase gene, may also be required for the <italic>aaftsH</italic> interference. This is the first report of RNase III‐dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.</p> </sec> </abstract>