Establishment of TSH β real‐time monitoring system in mammalian photoperiodism. (12th June 2013)

• Record Type: Journal Article
• Authors: Tsujino, Kaori; Narumi, Ryohei; Masumoto, Koh‐hei; Susaki, Etsuo A.; Shinohara, Yuta; Abe, Takaya; Iigo, Masayuki; Wada, Atsushi; Nagano, Mamoru; Shigeyoshi, Yasufumi; Ueda, Hiroki R.
• Is Part Of: Genes to cells. Volume 18:Number 7(2013:Jul.)
• Page Start: 575
• Page End: 588
• Publication Date: 2013-06-12
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12063
• Languages: English
• ISSNs: 1356-9597

Abstract

<abstract abstract-type="main" id="gtc12063-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Organisms have seasonal physiological changes in response to day length. Long‐day stimulation induces thyroid‐stimulating hormone beta subunit (<italic>TSH</italic>β) in the pars tuberalis (PT), which mediates photoperiodic reactions like day‐length measurement and physiological adaptation. However, the mechanism of <italic>TSH</italic>β induction for day‐length measurement is largely unknown. To screen candidate upstream molecules of <italic>TSH</italic>β, which convey light information to the PT, we generated <italic>Luciferase</italic> knock‐in mice, which quantitatively report the dynamics of <italic>TSH</italic>β expression. We cultured brain slices containing the PT region from adult and neonatal mice and measured the bioluminescence activities from each slice over several days. A decrease in the bioluminescence activities was observed after melatonin treatment in adult and neonatal slices. These observations indicate that the experimental system possesses responsiveness of the <italic>TSH</italic>β expression to melatonin. Thus, we concluded that our experimental system monitors <italic>TSH</italic>β expression dynamics in response to external stimuli.</p> </abstract>