Intact Long‐Type dupA as a Marker for Gastroduodenal Diseases in Okinawan Subpopulation, Japan. Issue 1 (22nd August 2012)

• Record Type: Journal Article
• Authors: Takahashi, Ayaka; Shiota, Seiji; Matsunari, Osamu; Watada, Masahide; Suzuki, Rumiko; Nakachi, Saori; Kinjo, Nagisa; Kinjo, Fukunori; Yamaoka, Yoshio
• Is Part Of: Helicobacter. Volume 18:Issue 1(2013:Feb.)
• Page Start: 66
• Page End: 72
• Publication Date: 2012-08-22
• Subjects: Helicobacter -- Periodicals; Helicobacter infections -- Periodicals; Stomach -- Diseases -- Periodicals; 616.3301405
• DOI: 10.1111/j.1523-5378.2012.00994.x
• Languages: English
• ISSNs: 1083-4389

Abstract

<abstract abstract-type="main" id="hel994-abs-0001"> <title>Abstract</title> <sec id="hel994-sec-0001" sec-type="section"> <title>Background</title> <p> <italic>Helicobacter pylori dupA</italic> can be divided into two types according to the presence or absence of the mutation. In addition, full‐sequenced data revealed that <italic>dupA</italic> has two types with different lengths depend on the presence of approximately 600 bp in the putative 5′ region (presence; long‐type and absence; short‐type), which has not been taken into account in previous studies.</p> </sec> <sec id="hel994-sec-0002" sec-type="section"> <title>Methods</title> <p>A total of 319 strains isolated from Okinawa, the south islands of Japan, were included. The status of <italic>dupA</italic> and <italic>cagA</italic> was determined by polymerase chain reaction. The presence of mutations in long‐type <italic>dupA</italic> was determined by DNA sequencing.</p> </sec> <sec id="hel994-sec-0003" sec-type="section"> <title>Results</title> <p>The prevalence of long‐type <italic>dupA</italic> was 26.3% (84/319). Sequence analysis showed that there were only six cases (7.1%) with point mutations lead to stop codon among 84 long‐type <italic>dupA</italic> strains studied. Interestingly, intact long‐type <italic>dupA</italic> without frameshift mutation<italic>, </italic> but not short‐type <italic>dupA, </italic> was significantly associated with gastric ulcer and gastric cancer than gastritis (<italic>p</italic> = .001 and <italic>p</italic> = .019, respectively). After adjustment by age, gender, and <italic>cagA</italic>, the presence of intact long‐type <italic>dupA</italic> was significantly associated with gastric ulcer and gastric cancer compared with gastritis (odds ratio [OR] = 3.35, 95% confidence interval [CI] = 1.55–7.24 and OR = 4.14, 95% CI = 1.23–13.94, respectively).</p> </sec> <sec id="hel994-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Intact long‐type <italic>dupA</italic> is a real virulence marker for severe outcomes in Okinawa, Japan. The previous information gained from PCR‐based methods without taking long‐type <italic>dupA</italic> into account must be interpreted with caution.</p> </sec> </abstract>