Grateloupia lanceolata (Okamura) Kawaguchi, the Edible Red Seaweed, Inhibits Lipid Accumulation and Reactive Oxygen Species Production During Differentiation in 3T3‐L1 Cells. (29th June 2012)

• Record Type: Journal Article
• Authors: Seo, Min‐Jung; Choi, Hyeon‐Son; Lee, Ok‐Hwan; Lee, Boo‐Yong
• Is Part Of: Phytotherapy research. Volume 27:Number 5(2013:May)
• Page Start: 655
• Page End: 663
• Publication Date: 2012-06-29
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.4765
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Grateloupia lanceolata</italic> (Okamura) Kawaguchi is a red alga native to coastal areas of East Asia. The effect of a <italic>G. lanceolata</italic> extract on lipid accumulation and reactive oxygen species (ROS) production in 3T3‐L1 cells was assessed by examining adipogenic transcription factors and ROS‐regulating genes at the molecular level. An ethanol extract of <italic>G. lanceolata</italic> inhibited lipid accumulation and ROS production during adipogenesis. Treatment with the <italic>G. lanceolata</italic> extract lead to a reduction in the mRNA levels of the transcription factors, peroxisome proliferator‐activated receptor‐γ and CCAAT/ enhancer binding protein‐α, and at the protein level for the target protein, adipocyte protein 2. ROS‐producing nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase 4 and NADPH‐producing glucose‐6‐phosphate dehydrogenase mRNAs decreased following <italic>G. lanceolata</italic> extract treatment. In contrast, the mRNA level of ROS scavenging enzymes, including superoxide dismutase (SOD), glutathione peroxidase, and catalase increased in the extract‐treated group. The increase in SOD1 (Cu/Zn‐SOD) and 2 (Mn‐SOD) proteins was correlated with their mRNA levels. Additionally, the <italic>G. lanceolata</italic> extract significantly enhanced mRNA levels of adiponectin, one of the adipokines secreted from adipocytes. Our results show that <italic>G. lanceolata</italic> extract inhibited lipid accumulation and ROS production by controlling adipogenic signals and ROS regulating genes. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>