DNA repair and replication proteins as prognostic markers in melanoma. Issue 2 (28th September 2012)

• Record Type: Journal Article
• Authors: Song, Liang; Robson, Tammy; Doig, Tamasin; Brenn, Thomas; Mathers, Marie; Brown, Ewan R; Doherty, Val; Bartlett, John M S; Anderson, Niall; Melton, David W
• Is Part Of: Histopathology. Volume 62:Issue 2(2013)
• Page Start: 343
• Page End: 350
• Publication Date: 2012-09-28
• Subjects: Histology, Pathological -- Periodicals; 611.018
• DOI: 10.1111/j.1365-2559.2012.04362.x
• Languages: English
• ISSNs: 0309-0167

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Aims: </bold> Elevated expression of DNA repair and replication genes has been reported in thick, non‐fixed primary melanomas that subsequently went on to metastasize, when compared to non‐recurrent primary tumours. This increased expression could contribute to the extreme resistance shown by melanoma to DNA‐damaging chemotherapeutics. We have investigated the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in melanoma.</p> <p> <bold>Methods and results: </bold> We used a tissue microarray containing samples from all stages of melanomagenesis to investigate the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in a larger, more representative and readily available set of fixed primary melanomas. High expression of topoisomerase IIα (TOP2A), that relieves torsional stress during DNA replication, and XRCC5 (Ku80), required for DNA double‐strand break repair, were associated with significantly worse survival.</p> <p> <bold>Conclusions: </bold> Two (XRCC5 and TOP2A) of seven DNA repair and replication proteins studied were prognostic for melanoma.</p> </abstract>