Increased expression of phospholipase Dα1 in guard cells decreases water loss with improved seed production under drought in Brassica napus. Issue 3 (29th December 2012)

• Record Type: Journal Article
• Authors: Lu, Shaoping; Bahn, Sung C.; Qu, Gang; Qin, Haiying; Hong, Yue; Xu, Qingpeng; Zhou, Yongming; Hong, Yueyun; Wang, Xuemin
• Is Part Of: Plant biotechnology journal. Volume 11:Issue 3(2013:Apr.)
• Page Start: 380
• Page End: 389
• Publication Date: 2012-12-29
• Subjects: Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12028
• Languages: English
• ISSNs: 1467-7644

Abstract

<abstract abstract-type="main" id="pbi12028-abs-0001"> <title>Summary</title> <p>The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in <italic>Arabidopsis</italic>. To explore the use of the PLDα1‐mediated signalling towards decreasing water loss in crop plants, we introduced <italic>Arabidopsis PLDα1</italic> under the control of a guard cell–specific promoter <italic>AtKatI</italic>_{<italic>pro</italic>} into two canola (<italic>Brassica napus</italic>) cultivars. Multiple <italic>AtKatI</italic>_{<italic>pro</italic>}<italic>::PLDα1</italic> lines in each cultivar displayed decreased water loss and improved biomass accumulation under hyperosmotic stress conditions, including drought and high salinity. Moreover, <italic>AtKatI</italic>_{<italic>pro</italic>}<italic>::PLDα1</italic> plants produced more seeds than did WT plants in fields under drought. The results indicate that the guard cell–specific expression of <italic>PLDα1</italic> has the potential to improve crop yield by enhancing drought tolerance.</p> </abstract>