Transcriptional control of lipid metabolism by the MarR‐like regulator FamR and the global regulator GlxR in the lipophilic axilla isolate Corynebacterium jeikeium K411. Issue 2 (20th November 2012)

• Record Type: Journal Article
• Authors: Barzantny, Helena; Guttmann, Sarah; Lässig, Charlotte; Brune, Iris; Tauch, Andreas
• Is Part Of: Microbial biotechnology. Volume 6:Issue 2(2013:Mar.)
• Page Start: 118
• Page End: 130
• Publication Date: 2012-11-20
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.12004
• Languages: English
• ISSNs: 1751-7915

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Corynebacterial fatty acid metabolism has been associated with human body odour, and is therefore discussed as a potential target for the development of new deodorant additives. For this reason, the transcription levels of <italic>fad</italic> genes associated with lipid metabolism in the axilla isolate <italic>Corynebacterium jeikeium</italic> were analysed during growth on different lipid sources. The transcription of several <italic>fad</italic> genes was induced two‐ to ninefold in the presence of Tween 60, including the acyl‐CoA dehydrogenase gene <italic>fadE6</italic>. DNA affinity chromatography identified the MarR‐like protein FamR as candidate regulator of <italic>fadE6</italic>. DNA band shift assays and <italic>in vivo</italic> reporter gene fusions confirmed the direct interaction of FamR with the mapped <italic>fadE6</italic> promoter region. Moreover, DNA affinity chromatography and DNA band shift assays detected the binding of GlxR to the promoter regions of <italic>fadE6</italic> and <italic>famR</italic>, revealing a hierarchical control of <italic>fadE6</italic> transcription by a feed‐forward loop. Binding of GlxR and FamR to additional <italic>fad</italic> gene regions was demonstrated <italic>in vitro</italic> by DNA band shift assays, resulting in the co‐regulation of <italic>fadA</italic>, <italic>fadD</italic>, <italic>fadE</italic> and <italic>fadH</italic> genes. These results shed first light on the hierarchical transcriptional control of lipid metabolism in <italic>C. jeikeium</italic>, a pathway associated with the development of human axillary odour.</p> </abstract>