Flavonol‐containing phosphorylated pullulan may attenuate pulp inflammation. (7th July 2012)

• Record Type: Journal Article
• Authors: Yonehiro, J.; Yoshida, Y.; Yamashita, A.; Yoshizawa, S.; Ohta, K.; Kamata, N.; Okihara, T.; Nishimura, F.
• Is Part Of: International endontic journal. Volume 46:Number 2(2013:Feb.)
• Page Start: 119
• Page End: 127
• Publication Date: 2012-07-07
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/j.1365-2591.2012.02095.x
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" xml:lang="en" id="iej2095-abs-0001"> <title>Abstract</title> <sec id="iej2095-sec-0001" sec-type="section"> <title>Aim</title> <p>To find possible reagents to minimize inflammatory responses by using an established pulpitis models for the purpose of developing new pulp‐capping materials, and to test the possible use of phosphorylated pullulan as a carrier for such an anti‐inflammatory reagent.</p> </sec> <sec id="iej2095-sec-0002" sec-type="section"> <title>Methodology</title> <p>Co‐culturing was performed using transwell systems. Inflammatory responses were evaluated by measuring cytokines produced by the cells. The effects of two flavonoids, luteolin and quercetin, as anti‐inflammatory reagents, and phosphorylated pullulan, which potentially achieves a sufficient marginal sealing to hydroxyapatite and slowly releases luteolin, as a carrier for flavonoids, were tested.</p> </sec> <sec id="iej2095-sec-0003" sec-type="section"> <title>Results</title> <p>Flavonols, particularly luteolin, dramatically attenuated inflammatory cytokine production, which was augmented by co‐cultures. Luteolin was successfully enclosed by phosphorylated pullulan. Finally, it was confirmed that luteolin released from phosphorylated pullulan was effective in reducing cytokine production by co‐cultures.</p> </sec> <sec id="iej2095-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Combination of phosphorylated pullulan and luteolin could be potentially used in the treatment of dental pulp inflammation.</p> </sec> </abstract>