Osteogenic differentiation of adipose‐derived stem cells prompted by low‐intensity pulsed ultrasound. (22nd May 2013)

• Record Type: Journal Article
• Authors: Yue, Y.; Yang, X.; Wei, X.; Chen, J.; Fu, N.; Fu, Y.; Ba, K.; Li, G.; Yao, Y.; Liang, C.; Zhang, J.; Cai, X.; Wang, M
• Is Part Of: Cell proliferation. Volume 46:Number 3(2013:Jun.)
• Page Start: 320
• Page End: 327
• Publication Date: 2013-05-22
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12035
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" xml:lang="en" id="cpr12035-abs-0001"> <title>Abstract</title> <sec id="cpr12035-sec-0001" sec-type="section"> <title>Objectives</title> <p>Based on <italic>in vivo</italic> studies, low‐intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non‐union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters.</p> </sec> <sec id="cpr12035-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real‐time PCR, western blotting and immunofluorescence.</p> </sec> <sec id="cpr12035-sec-0003" sec-type="section"> <title>Results</title> <p>Our data indicated that LIPUS promoted mRNA levels of runt‐related transcription factor 2, osteopontin and osterix in the presence of osteo‐induction medium; moreover, protein levels of runt‐related transcription factor 2 and osteopontin were upregulated.</p> </sec> <sec id="cpr12035-sec-0004" sec-type="section"> <title>Conclusions</title> <p>We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.</p> </sec> </abstract>