Managing outbreaks of the citrus long‐horned beetle Anoplophora chinensis (Forster) in Europe: molecular diagnosis of plant infestation. Issue 5 (24th October 2012)

• Record Type: Journal Article
• Authors: Strangi, Agostino; Sabbatini Peverieri, Giuseppino; Roversi, Pio Federico
• Is Part Of: Pest management science. Volume 69:Issue 5(2013:May)
• Page Start: 627
• Page End: 634
• Publication Date: 2012-10-24
• Subjects: Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.3416
• Languages: English
• ISSNs: 1526-498X

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="ps3416-sec-0001" sec-type="section"> <title>Background</title> <p> <bold> <italic>Anoplophora chinensis</italic> (Forster) (Coleoptera Cerambycidae) is a polyphagous long‐horned beetle native to Eastern Asia that infests a wide range of broadleaved plants, causing disruption of vascular tissue, structural weakness and tree death. As a result, <italic>A. chinensis</italic> is a quarantine pest for the European Union. In order to confirm its infestations on plants, it is necessary to obtain adult or larval specimens on which morphological or molecular analysis can be performed. However, obtaining such specimens from infested plants can be a demanding and difficult task. Therefore, a diagnostic tool that is non‐invasive for the plant and able to be performed in the absence of any insect stages may be useful to confirm infestations of <italic>A. chinensis</italic> on plants.</bold> </p> </sec> <sec id="ps3416-sec-0002" sec-type="section"> <title>Results</title> <p> <bold>A protocol is presented that is based on polymerase chain reaction amplification using DNA extracted from <italic>A. chinensis</italic> faecal material collected in the field.</bold> </p> </sec> <sec id="ps3416-sec-0003" sec-type="section"> <title>Conclusion</title> <p> <bold>Results obtained in the present work show that the non‐invasive approach is a reliable and accurate alternative diagnostic tool in phytosanitary surveys.© 2012 Society of Chemical Industry</bold> </p> </sec> </abstract>