Immunoregulatory profile of monocytes from cutaneous leishmaniasis patients and association with lesion size. (9th January 2013)

• Record Type: Journal Article
• Authors: Vieira, É. L. M.; Keesen, T. S. L.; Machado, P. R.; Guimarães, L. H.; Carvalho, E. M.; Dutra, W. O.; Gollob, K. J.
• Is Part Of: Parasite immunology. Volume 35:Number 2(2013:Feb.)
• Page Start: 65
• Page End: 72
• Publication Date: 2013-01-09
• Subjects: Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12012
• Languages: English
• ISSNs: 0141-9838

Abstract

<abstract abstract-type="main" xml:lang="en" id="pim12012-abs-0001"> <title>Summary</title> <p>Leishmaniasis is an important tropical disease composed of several clinical forms that adversely affect millions of people globally. Critical cells involved in the host–<italic>Leishmania</italic> interaction are monocytes and macrophages, which act to protect against infections due to their ability to both control intracellular infections and regulate the subsequent adaptive immune response. Both soluble factors and cell surface receptors are keys in directing the immune response following interaction with pathogens such as <italic>Leishmania</italic>. Toll‐like receptors (TLRs) have an essential role in immune responses against infections, but little is known about their role in human infection with <italic>Leishmania braziliensis</italic>. In this work, we evaluated peripheral blood CD14<sup>+</sup> monocytes for the expression of immunoregulatory cytokines, co‐stimulatory molecules and TLR9 from cutaneous leishmaniasis patients infected with <italic>L. braziliensis</italic> and noninfected individuals. Our results showed that patients present decreased expression of co‐stimulatory molecules such as CD80 and CD86 following culture with media alone or after stimulus with soluble <italic>Leishmania</italic> antigen. Interestingly, TLR9 expression was higher after culture with soluble <italic>Leishmania</italic> antigen (SLA), suggesting a role of this molecule in immunoregulation of active disease. Lastly, higher frequencies of TLR9<sup>+</sup> monocytes were correlated with greater lesion size. These findings demonstrate a peripheral monocytes profile compatible with important immunoregulatory potential.</p> </abstract>