Spatiotemporal reconstruction of the Aquilegia rapid radiation through next‐generation sequencing of rapidly evolving cpDNA regions. Issue 2 (5th February 2013)

• Record Type: Journal Article
• Authors: Fior, Simone; Li, Mingai; Oxelman, Bengt; Viola, Roberto; Hodges, Scott A.; Ometto, Lino; Varotto, Claudio
• Is Part Of: New phytologist. Volume 198:Issue 2(2013)
• Page Start: 579
• Page End: 592
• Publication Date: 2013-02-05
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12163
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12163-abs-0001"> <title>Summary</title> <p> <list id="nph12163-list-0001" list-type="bullet"> <list-item> <p> <italic>Aquilegia</italic> is a well‐known model system in the field of evolutionary biology, but obtaining a resolved and well‐supported phylogenetic reconstruction for the genus has been hindered by its recent and rapid diversification.</p> </list-item> <list-item> <p>Here, we applied 454 next‐generation sequencing to PCR amplicons of 21 of the most rapidly evolving regions of the plastome to generate <italic>c</italic>. 24 kb of sequences from each of 84 individuals from throughout the genus.</p> </list-item> <list-item> <p>The resulting phylogeny has well‐supported resolution of the main lineages of the genus, although recent diversification such as in the European taxa remains unresolved. By producing a chronogram of the whole Ranunculaceae family based on published data, we inferred calibration points for dating the <italic>Aquilegia</italic> radiation. The genus originated in the upper Miocene <italic>c</italic>. 6.9 million yr ago (Ma) in Eastern Asia, and diversification occurred <italic>c</italic>. 4.8 Ma with the split of two main clades, one colonizing North America, and the other Western Eurasia through the mountains of Central Asia. This was followed by a back‐to‐Asia migration, originating from the European stock using a North Asian route.</p> </list-item> <list-item> <p>These results provide the first backbone phylogeny and spatiotemporal reconstruction of the <italic>Aquilegia</italic> radiation, and constitute a robust framework to address the adaptative nature of speciation within the group.</p> </list-item> </list> </p> </abstract>