Hydrogen peroxide‐regulated genes in the Medicago truncatula–Sinorhizobium meliloti symbiosis. Issue 1 (24th January 2013)

• Record Type: Journal Article
• Authors: Andrio, Emilie; Marino, Daniel; Marmeys, Anthony; de, Marion Dunoyer; Damiani, Isabelle; Genre, Andrea; Huguet, Stéphanie; Frendo, Pierre; Puppo, Alain; Pauly, Nicolas
• Is Part Of: New phytologist. Volume 198:Issue 1(2013)
• Page Start: 179
• Page End: 189
• Publication Date: 2013-01-24
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12120
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12120-abs-0001"> <title>Summary</title> <p> <list id="nph12120-list-0001" list-type="bullet"> <list-item> <p>Reactive oxygen species (ROS), particularly hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), play an important role in signalling in various cellular processes. The involvement of H<sub>2</sub>O<sub>2</sub> in the <italic>Medicago truncatula</italic>–<italic>Sinorhizobium meliloti</italic> symbiotic interaction raises questions about its effect on gene expression.</p> </list-item> <list-item> <p>A transcriptome analysis was performed on inoculated roots of <italic>M. truncatula</italic> in which ROS production was inhibited with diphenylene iodonium (DPI). In total, 301 genes potentially regulated by ROS content were identified 2 d after inoculation. These genes included <italic>MtSpk1</italic>, which encodes a putative protein kinase and is induced by exogenous H<sub>2</sub>O<sub>2</sub> treatment.</p> </list-item> <list-item> <p> <italic>MtSpk1</italic> gene expression was also induced by nodulation factor treatment. <italic>MtSpk1</italic> transcription was observed in infected root hair cells, nodule primordia and the infection zone of mature nodules. Analysis with a fluorescent protein probe specific for H<sub>2</sub>O<sub>2</sub> showed that <italic>MtSpk1</italic> expression and H<sub>2</sub>O<sub>2</sub> were similarly distributed in the nodule infection zone. Finally, the establishment of symbiosis was impaired by <italic>MtSpk1</italic> downregulation with an artificial micro‐RNA.</p> </list-item> <list-item> <p>Several genes regulated by H<sub>2</sub>O<sub>2</sub> during the establishment of rhizobial symbiosis were identified. The involvement of <italic>MtSpk1</italic> in the establishment of the symbiosis is proposed.</p> </list-item> </list> </p> </abstract>