An acute reversible experimental model of pneumonia in pigs: time‐related histological and clinical signs changes. Issue 3 (28th May 2012)

• Record Type: Journal Article
• Authors: VILLARINO, N.; GARCIA‐TAPIA, D.; LESMAN, S.; LUCAS, M.; ROBINSON, J.; BROWN, S. A.; MARTIN‐JIMENEZ, T.
• Is Part Of: Journal of veterinary pharmacology and therapeutics. Volume 36:Issue 3(2013)
• Page Start: 241
• Page End: 247
• Publication Date: 2012-05-28
• Subjects: Veterinary pharmacology -- Periodicals; Therapeutics -- Periodicals; 636.0895105
• DOI: 10.1111/j.1365-2885.2012.01415.x
• Languages: English
• ISSNs: 0140-7783

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The objective of this study was to evaluate the long‐term survival rates, clinical response, and lung gross and microscopic changes in pigs treated intratracheally with lipopolysaccharide of <italic>Escherichia coli</italic> 0111:B4 (LPS‐<italic>Ec</italic>). Healthy pigs were randomly allocated to three groups: (i) no‐LPS‐<italic>Ec</italic> (<italic>n</italic> = 1), (ii) LPS‐<italic>Ec</italic>‐T1 (1 mg/mL, 10 mL/pig) (<italic>n</italic> = 7), and (iii) LPS‐<italic>Ec</italic>‐T2 (0.5 mg/mL, 10 mL/pig) (<italic>n</italic> = 6). Two pigs from each dose group were euthanized at 24 (<italic>n</italic> = 3 for T1), 48 and 144 h post‐LPS‐<italic>Ec</italic> challenge. LPS<italic>‐Ec</italic>‐treated animals showed macroscopic lesions in middle lobes of the lung. A reversible recruitment of macrophages and neutrophils was observed at 24, 48, and 144 h post‐LPS‐<italic>Ec</italic> challenge. The highest cellular infiltration level was observed at 24 h after challenge. The highest clinical scores were evident in both experimental dose levels within 3 and 5 h after LPS‐<italic>Ec</italic> administration. Administration of LPS‐<italic>Ec</italic>, under the conditions evaluated, can be used to induce a reproducible model of acute pulmonary inflammation in pigs.</p> </abstract>