Comparison of C60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections. (2nd February 2012)

• Record Type: Journal Article
• Authors: Fletcher, John S.; Rabbani, Sadia; Barber, Andrew M.; Lockyer, Nicholas P.; Vickerman, John C.
• Is Part Of: Surface and interface analysis. Volume 45:Number 1(2013:Jan.)
• Page Start: 273
• Page End: 276
• Publication Date: 2012-02-02
• Subjects: Surfaces (Physics) -- Periodicals; Surface chemistry -- Periodicals; Thin films -- Periodicals; 541.33
• DOI: 10.1002/sia.4874
• Languages: English
• ISSNs: 0142-2421

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>We have implemented a gas cluster ion beam (GCIB) system developed by Ionoptika Ltd (Southampton, UK) with sufficient control to allow us to exploit the unique capabilities of our J105 instrument for imaging and depth profiling. The J105 allows us to use the GCIB as continuous primary ion beam, thereby overcoming the issues associated with pulsing these slow moving, mixed species beams. We have performed a direct comparison with C<sub>60</sub> ions on the same samples in the same instrument. The GCIB beams are more difficult to focus than the C<sub>60</sub><sup>+</sup> ion beam, making single‐cell imaging difficult, although spot sizes of 15–20 µm are readily obtainable for Ar<sub>1000</sub> and Ar<sub>2000</sub>, providing good resolution for larger area imaging on tissue section/biopsy samples. In this paper, we present results from the assessment of these new beams as primary ions for the analysis of 'real', complex biological systems. Initial spectra and those following increased primary ion bombardment were compared for <italic>in vitro</italic> cultured cells deposited on silicon and cryo‐sectioned tumour samples originating <italic>in vivo</italic>. Ar<sub>1000</sub><sup>+</sup> and Ar<sub>2000</sub><sup>+</sup> showed increased persistence of the signals from intact molecular ions of phospholipids and a reduction in the accumulation of chemical background noise compared with C<sub>60</sub><sup>+</sup> analysis. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>