Integrated methods in protein biochemistry. Part C (2023)

• Record Type: Book
• Further Information: Note: Editor, Arun K. Shukla.
• Editors: Shukla, Arun K
• Contents: 1. NanoBiT-based methods to monitor the activation and modulation of RTKs; 2. The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling; 3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems; 4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase; 5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase; 6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms; 7. Covalent protein painting: MS-based protein footprinting; 8. Characterization of GPCR signaling complexes using negative-staining electron microscopy; 9. Probing protein misfolding and dissociation with free electron laser; 10. Optimized protocol for the characterization of Cas12a activities; 11. Proximity proteomics for the identification and characterization of extracellular vesicles; 12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs); 13. Characterization of RRE domain in RiPP biosynthesis; 14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations; 15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging; 16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry; 17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins; 18. Quantitative cysteinome profiling with DIA-ABPP; 19. High-throughput screening and evolution of carbohydrate-active enzymes; 20. Regulation of enzymes with light using unnatural amino acids; 21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies; 22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics; 23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors; 24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules; 25. Reconstitution of the MLL1 complex and probing methyltransferase activity; 26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy; 27. Structural and functional analysis of mono-copper enzymes from fungal species; 28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins; 29. Electrophysiology of ion-transporting rhodopsins; 30. Measuring protein post-translational modifications using the protein semi-synthesis; 31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2); 32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP); 33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry; 34. Global substrate profiling of post-translational modifying enzymes; 35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide
• Publisher Details: Amsterdam : Academic Press
• Publication Date: 2023
• Extent: 1 online resource (412 pages)
• Subjects: 572.6; Proteins -- Analysis -- Research -- Methodology; Proteomics -- Methodology; Biochemistry
• Languages: English
• ISBNs: 9780443185939
• Related ISBNs: 9780443185922
• Notes: Note: Description based on CIP data; resource not viewed.
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