Integrated methods in protein biochemistry. Part B (2023)

• Record Type: Book
• Further Information: Note: Editor, Arun K. Shukla.
• Editors: Shukla, Arun K
• Contents: 1. Precise modification of native proteins, purification, and analysis of bioconjugates; 2. NanoBiT-based methods to monitor the activation and modulation of RTKs; 3. The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling; 4. Structure and function of bacterial secretion system; 5. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase; 6. Tools and protocols for probing protein sumoylation; 7. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase; 8. DeGlyPHER: MS-based analysis of viral spike N-glycoforms; 9. Covalent protein painting: MS-based protein footprinting; 10. Characterization of GPCR signaling complexes using negative-staining electron microscopy; 11. Probing protein misfolding and dissociation with free electron laser; 12. Optimized protocol for the characterization of Cas12a activities; 13. Proximity proteomics for the identification and characterization of extracellular vesicles; 14. Structural and Functional characterization of lytic polysaccharide monooxygenases; 15. Characterization of RRE domain in RiPP biosynthesis; 16. Structural and mechanistic insights into ferrous iron import and export systems; 17. Testing anti-cancer drugs with holographic Incoherent light source quantitative phase imaging; 18. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry; 19. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins; 20. Integrated studies on ageing-related proteins extending life span in yeast; 21. An optimized pipeline for global selenoproteome analysis; 22. High-throughput screening and evolution of carbohydrate-active enzymes; 23. Regulation of enzymes with light using unnatural amino acids; 24. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies; 25. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics; 26. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors; 27. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules; 28. Reconstitution of the MLL1 complex and probing methyltransferase activity; 29. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy; 30. Structural and functional analysis of mono-copper enzymes from fungal species
• Publisher Details: Amsterdam : Academic Press
• Publication Date: 2023
• Extent: 1 online resource
• Subjects: 572.6; Proteins -- Analysis -- Research -- Methodology; Proteomics -- Methodology; Biochemistry
• Languages: English
• ISBNs: 9780323992657
• Related ISBNs: 9780323992640
• Notes: Note: Description based on CIP data; resource not viewed.
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