Carotenoids : carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology /: carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. (2022)

• Record Type: Book
• Further Information: Note: Editor, Eleanore Wurtzel.
• Editors: Wurtzel, Eleanore Tamar
• Contents: 1. Metabolomics-based analysis of carotenoids and related metabolites in various species via quantitative trait loci and genome wide association mapping approaches; 2. Enzymatic isomerization of ?-carotene mediated by the heme-containing isomerase Z-ISO; 3. Using bacteria for functional analysis of genes encoding carotenoid biosynthetic enzymes; 4. Rice Callus as a High Throughput Platform for Synthetic Biology and Metabolic Engineering of Carotenoids; 5. Transient expression in Nicotiana benthamiana: A simple platform to investigate genes encoding carotenoid biosynthesis enzymes from diverse algal lineages; 6. Protein-protein interaction techniques to investigate post-translational regulation of carotenogenesis; 7. The isolation of sub-chromoplast structures from tomato and capsicum fruit; 8. Carrot protoplasts as a suitable method for protein subcellular localization; 9. High throughput production and characterization of carotenoid enzymes for structural and functional studies; 10. Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation; 11. Characterizing CYTOCHROME P450 Enzymes Involved in Plant Apocarotenoid Metabolism by Using Engineered Yeast System; 12. Preparation of carotenoid cleavage dioxygenases for crystallography; 13. Heme-dependent enzymes involved in carotenoids biosynthesis in plants; 14. Tracking sub-plastidic localization of carotenoid metabolic enzymes with proteomics; 15. Golden Rice and lessons learned for inspiring future metabolic engineering strategies and synthetic biology solutions; 16. Genetic transformation of Cassava to improve carotenoids: a protocol revisited; 17. Elevating fruit carotenoid content in apple (Malus x domestica Borkh); 18. Engineering microbial synthesis of carotenoids; 19. The breeder's tool-box for enhancing the content of esterified carotenoids in wheat: from extraction and profiling of carotenoids to marker-assisted selection of candidate genes; 20. Drawing on enzyme promiscuity to create novel carotenoids via non-phytoene precursors; 21. Plant abscisic acid receptor agonists and antagonists; 22. A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi; 23. Designing biosensors from a plant apocarotenoid receptor; 24. Imaging Retinaldehyde-Protein Binding in Plants using a Merocyanine Reporter 25. Use of directed enzyme evolution to create novel biosynthetic pathways for production of non-natural and rare carotenoids
• Publisher Details: Amsterdam : Academic Press
• Publication Date: 2022
• Extent: 1 online resource
• Subjects: 572.59; Carotenoids -- Biotechnology; Carotenoids -- Analysis
• Languages: English
• ISBNs: 9780323913546
• Related ISBNs: 9780323913539
• Notes: Note: Description based on CIP data; resource not viewed.
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