Phagocytosis and Phagosomes : Methods and Protocols /: Methods and Protocols. (2016)

• Record Type: Book
• Further Information: Note: Roberto Botelho.
• Editors: Botelho, Roberto
• Contents: Phagocytosis: Hungry, Hungry Cells.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantitative Efferocytosis Assays.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Single Cell Analysis of Phagocytosis, Phagosome Maturation, Phagolysosomal Leakage and Cell Death Following Exposure of Macrophages to Silica Particles.- Quantitative Live-cell Fluorescence Microscopy during Phagocytosis.- Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers.- Quantitative Immunofluorescence to Study Phagosome Maturation.- Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho Gtpase Activation during Phagocytosis.- Analysis of LC3-associated Phagocytosis and Antigen Presentation.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measuring Phagosomal pH by Fluorescence Microscopy.- Image-Based Analysis of Phagocytosis – Measuring Engulfment and Internalization.- Fluorometric Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Simultaneous Analysis of Multiple Lumenal Parameters of Individual Phagosomes Using High-Content Imaging.- <isolation and="" western="" blotting="" of="" latex-bead="" phagosomes="" to="" track="" phagosome="" maturation.-="" assessing="" the="" proteome="" by="" quantitative="" mass="" spectrometry.-="" dissecting="" phagocytic="" removal="" apoptotic="" cells="" in="" Caenorhabditis Elegans.- Measurement of Salmonella Enterica Internalization and Vacuole Lysis in Epithelial Cells.- Bacterial Binding, Phagocytosis and Killing: Measurements Using Colony Forming Units.- Filamentous Bacteria as Targets to Study Phagocytosis.- Growing and Handling of Mycobacterium Tuberculosis for Macrophage Infection Assays.- Mycobacterium Tuberculosis: Readouts of Bacterial Fitness and The Environment within the Phagosome.- Using Flow Cytometry to Analyse Cryptococcus Infection in Macrophages.>.
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Copyright Date: 2017
• Extent: 1 online resource (360 pages)
• Subjects: Life sciences; Microbiology; Science -- Life Sciences -- Biology -- Microbiology; Microbiology (non-medical)
• Languages: English
• ISBNs: 9781493965816
• Related ISBNs: 9781493965793
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.