Zebrafish : Methods and Protocols /: Methods and Protocols. (2016)

• Record Type: Book
• Further Information: Note: Koichi Kawakami, E. Elizabeth Patton, Michael Orger.
• Editors: Kawakami, Koichi; Patton, E. Elizabeth; Orger, Michael
• Contents: Chemical Screening in Zebrafish.- TALEN-Mediated Mutagenesis and Genome Editing.- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome.- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for the Study of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models.- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During Zebrafish Development.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.
• Edition: Second edition
• Publisher Details: New York : Humana Press
• Publication Date: 2016
• Copyright Date: 2016
• Extent: 1 online resource (373 pages)
• Subjects: Life sciences; Biochemistry; Science -- Life Sciences -- Biochemistry; Biochemistry
• Languages: English
• ISBNs: 9781493937714
• Related ISBNs: 9781493937691
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.