NF-kappa B : Methods and Protocols /: Methods and Protocols. (2015)

• Record Type: Book
• Further Information: Note: Michael J. May.
• Editors: May, Michael J
• Contents: Part I: Standard Approaches to Detect NF-κB Pathway1. Electrophoretic Mobility Shift Assay Analysis of NF-kB DNA BindingSitharam Ramaswami and Matthew S. Hayden M.D 2. Detection of IkB Degradation Dynamics and IkB-a Ubiquitination Petro Starokadomskyy and Ezra Burstein 3. Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-regulatory Elements using Luciferase AssaysPatricia E. Collins, Christine O'Carroll, and Ruaidhrí J. Carmody Part II: Detection and Analysis of NF-κB Signaling4. Assessing Sites of NF-κB DNA Binding using Chromatin ImmunoprecipitationAmy Colleran, Patricia E. Collins, and Ruaidhrí J. Carmody 5. IKK Kinase Assay for Assessment of Canonical NF-kB Activation in NeuronsAnca B Mihalas and Mollie K Meffert 6. Analysis of Epidermal Growth Factor-induced NF-kB Signaling Changying Jiang and Xin Lin 7. Methods to Assess the Activation of the Alternative (non-canonical) NF-kB Pathway by Non-death TNF ReceptorsCaroline Remouchamps and Emmanuel Dejardin 8. Systematic Detection of Non-canonical NF-κB ActivationZhaoxia Qu and Gutian Xiao 9. Non-canonical NF-κB activation and SDF-1 expression in Human Endothelial CellsKelly A. McCorkell and Michael J. May 10. Stable Reconstitution of IKK-deficient Mouse Embryonic Fibroblasts Carolyn M. Gray and Michael J. May 11. Dissecting NF-κB Signaling Induced by Genotoxic Agents Via Genetic Complementation of NEMO-deficient 1.3E2 CellsShawn S. Jackson and Shigeki Miyamoto Part III: Methods to Study the Control of NF-κB Signalling12. Visualizing TCR induced POLKADOTS Formation and NF-κB Activation in the D10 T Cell Clone and Mouse Primary Effector T cellsSuman Paul and Brian C Schaefer 13. Detection of Recombinant and Cellular MALT1 Paracaspase Activity Daniel Nagel and Daniel Krappmann 14. TRAF Protein Function In Non-Canonical NF-κB Signaling Anna D. Reichardt, Jose Pindado Rodriguez, Shivam A. Zaver, Genhong Cheng 15. Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-kB SignalingEugene Varfolomeev, Tatiana Goncharov, and Domagoj Vucic 16. Elucidating Dynamic Protein-protein Interactions and Ubiquitination in NF-kB Signaling PathwaysNoula Shembade and Edward W. Harhaj 17. Immunoblot Analysis of Linear Polyubiquitination of NEMOYoshiteru Sasaki, Hiroaki Fujita, Misa Nakai, and Kazuhiro Iwai 18. In vitro Detection of NEMO-Ubiquitin Binding using DELFIA and Microscale Thermophoresis AssaysMichelle Vincendeau, Daniel Krappmann, and Kamyar Hadian 19. Use of Fluorescence Spectroscopy For Quantitative Investigations Of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMOVirginie Dubosclard, Elisabeth Fontan, and Fabrice Agou 20. Generation of a Proteolytic Signal: E3/E2-mediated Polyubiquitination of IκBαRobert A. Chong, Kenneth Wu, Jordan Kovacev, and Zhen-Qiang Pan 21. Control of NF-κB Subunits by UbiquitinationPatricia E. Collins, Amy Colleran, and Ruaidhrí J. Carmody 22. Methodology to Study NF-kB/RelA Ubiquitination in vivoHaiying Li, Petro Starokadomskyy, and Ezra Burstein 23. Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-kB Tao Lu and George R. Stark 24. Methods to Detect NF-kB Acetylation and MethylationJinJing Chen and Lin-Feng Chen Part IV : Analyzing and Targeting NF-κB Activity in Disease25. A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 subunit of NF-kB from Patient-Derived Dermal Fibroblasts Alex W. Wessel and Eric P. Hanson 26. Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related ProteinsThomas D. Gilmore and Céline Gélinas 27. Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway Daniela S. Bassères and Albert S. Baldwin28. Immunohistochemical Analysis of NF-kB in Human Tumor TissueClint T. Allen and Carter Van Waes 29. Assessment of Canonical NF-kB Activity in Canine Diffuse Large B-Cell LymphomaAnita Gaurnier-Hausser and Nicola J. Mason 30. NEMO Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo Kelly A. McCorkell and Michael J. May 31. Regulation of NFκB Signaling in Osteoclasts and Myeloid ProgenitorsGaurav Swarnkar and Yousef Abu-Amer 32. NF-κB Activation with Aging: Characterization and Therapeutic Inhibition Jing Zhao, Xuesen Li, Sara McGowan, Laura J. Niedernhofer, and Paul D. Robbins 33. The 'Sneaking-ligand' Approach: Cell-type Specific Inhibition of the Classical NF-kB Pathway Bettina Sehnert, Harald Burkhardt, Stefan Dübel, and Reinhard E. Voll 34. Sneaking-ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-targeted NF-kB inhibition Bettina Sehnert, Harald Burkhardt, Michael J. May, Jochen Zwerina, and Reinhard E. Voll 35. Analysis of NF-kB Activation in Mouse Intestinal Epithelial CellsHelena Shaked, Monica Guma, and Michael Karin Part V: Bioinformatics and Modeling Approaches to Study NF-κB36. Characterizing the DNA Binding Site Specificity of NF-kB with Protein Binding Microarrays (PBMs) Trevor Siggers, Thomas D. Gilmore, Brian Barron, and Ashley Penvose 37. Methods for Analyzing the Evolutionary Relationship of NF-kB Proteins Using Free, Web-driven Bioinformatics and Phylogenetic ToolsJohn Finnerty and Thomas D. Gilmore 38. Studying NFkB Signaling with Mathematical ModelsSimon Mitchell, Rachel Tsui, and Alexander Hoffmann.
• Edition: 2015
• Publisher Details: New York : Humana Press
• Publication Date: 2015
• Copyright Date: 2015
• Extent: 1 online resource (668 pages)
• Subjects: Life sciences; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Biochemistry; Post-translational modification
• Languages: English
• ISBNs: 9781493924226
• Related ISBNs: 9781493924219
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.