Epigenetic reprogramming during mouse embryogenesis : methods and protocols /: methods and protocols. ([2021])

• Record Type: Book
• Further Information: Note: Edited by Katia Ancelin, Maud Borensztein.
• Other Names: Ancelin, Katia; Borensztein, Maud
• Contents: Preface… Table of Contents… Contributing Authors… 1. Ex Vivo Culture for Preimplantation Mouse Embryo to Analyse Pluripotency Katia Boutourlinsky, Nicolas Allègre, and Claire Chazaud 2. Microfabricated Device for High Resolution Imaging of Preimplantation Embryos Sandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, and Michel Cohen-Tannoudji 3. In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm Excision Hatice O. Ozguldez and Ivan Bedzhov 4. Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces Sophie M. Morgani and Anna-Katerina Hadjantonakis 5. Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines Malgorzata Borkowska and Harry G. Leitch 6. Generation of Primordial Germ Cell-Like Cells in Small and Large Scales Wolfram H. Gruhn and Ufuk Günesdogan 7. Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions Valentina Carlini, Kristjan H. Gretarsson, and Jamie A. Hackett 8. Somatic Reprogramming by Nuclear Transfer Vincent Brochard and Nathalie Beaujean 9. Targeted Transgenic Mice using CRISPR/Cas9 Technology Fatima El Marjou, Colin Jouhanneau, and Denis Krndija 10. Whole-Mount Immunofluorescence Staining of Early Mouse Embryos Frederick C. K. Wong 11. Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization Maud Borensztein 12. Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development Katia Ancelin, Yusuke Miyanari, Olivier Leroy, Maria-Elena Torres-Padilla, and Edith Heard 13. Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seq Raquel Pérez-Palacios, Patricia Fauque, Aurélie Teissandier, and Déborah Bourc'his 14. Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in Mammals Ambre Bender, Hala Al Adhami, Thomas Dahlet, and Michael Weber 15. Profiling DNA Methylation Genome-Wide in Single Cells Antonio Galvao and Gavin Kelsey 16. Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seq Bingjie Zhang, Xu Peng, Feng Xu, and Wei Xie 17. Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN Srinivasa Abishek Prakash and Joan Barau 18. DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse Embryos Mrinmoy Pal, Kim de Luca, Jop Kind, and Maria-Elena Torres-Padilla 19. Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C Analysis Noémie Ranisavljevic, Maud Borensztein, and Katia Ancelin 20. Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse Embryo Samuel Collombet, Yuvia A. Pérez-Rico, Katia Ancelin, Nicolas Servant, and Edith Heard Subject Index List....
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2021
• Extent: 1 online resource
• Subjects: 571.8/6; Embryology -- Laboratory manuals; Epigenetics -- Laboratory manuals; Genetics (non-medical); Biology, life sciences; Developmental biology; Science -- Life Sciences -- Genetics & Genomics; Science -- Life Sciences -- Biology -- General; Science -- Life Sciences -- Biology -- Developmental Biology; Electronic books
• Languages: English
• ISBNs: 9781071609583; 1071609580
• Related ISBNs: 1071609572; 9781071609576
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed November 11, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.