CRISPR guide RNA design : methods and protocols /: methods and protocols. ([2021])

• Record Type: Book
• Further Information: Note: Edited by Tudor A. Fulga, David J. H. F. Knapp, and Quentin R. V. Ferry.
• Other Names: Fulga, Tudor A; Knapp, David J. H. F; Ferry, Quentin R. V
• Contents: Part I: In Silico Design and Optimization of Guide RNA Sequences 1. Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments Florian Heigwer and Michael Boutros 2. Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer Gue-Ho Hwang, Jin-Soo Kim, and Sangsu Bae Part II: Chemically-Modified Guide RNAs 3. Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells Jenny Shapiro, Adi Tovin, Ortal Iancu, Daniel Allen, and Ayal Hendel 4. Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA Moira A. McMahon and Meghdad Rahdar 5. 'Split-and-Click' sgRNA Lapatrada Taemaitree, Arun Shivalingam, Afaf H. El-Sagheer, and Tom Brown 6. Chimeric DNA-RNA Guide RNA Designs Shuhan Lu, Ying Zhang, and Hao Yin Part III: Expanding the CRISPR Toolbox 7. Harnessing tRNA for Processing Ability and Promoter Activity David J.H.F. Knapp and Tudor A. Fulga 8. Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA–ncRNA Expression Constructs David M. Shechner 9. Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs Quentin R.V. Ferry 10. Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a Ha Rim Shin, Jiyeon Kweon, and Yongsub Kim Part IV: Characterization of CRISPR Efficacy and Specificity 11. Methods for Measuring CRISPR/Cas9 DNA Cleavage in Cells Christopher R. Cromwell, Juan Jovel, and Basil P. Hubbard 12. In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 Systems Christopher R. Cromwell and Basil P. Hubbard 13. Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq Daesik Kim and Jin-Soo Kim 14. Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector Xiaoling Wang, Youjun Wu, and Jiing-Kuan Yee 15. Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method Roberto Ballarino, Britta A.M. Bouwman, and Nicola Crosetto.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2021
• Extent: 1 online resource
• Subjects: 572.8/8; RNA editing -- Laboratory manuals; CRISPR (Genetics) -- Laboratory manuals; Biomedical engineering; Genetics (non-medical); Technology & Engineering -- Biomedical; Science -- Life Sciences -- Genetics & Genomics; Electronic books
• Languages: English
• ISBNs: 9781071606872; 1071606875
• Related ISBNs: 1071606867; 9781071606865
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed November 11, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.