Cardiac regeneration : methods and protocols /: methods and protocols. ([2021])

• Record Type: Book
• Further Information: Note: Edited by Kenneth D. Poss, Bernhard Kühn.
• Editors: Poss, Ken; Kühn, Bernhard
• Contents: Part I: Cardiac Injury Models 1. Myocardial Infarction Techniques in Adult Mice Elad Bassat, Dahlia Perez, and Eldad Tzahor 2. Apical Resection and Cryoinjury in Neonatal Mouse Heart Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien 3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt 4. Myocardial Infarction in Pigs Andrea Bähr, Nadja Hornaschewitz, and Christian Kupatt 5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish Ines J. Marques, Andrés Sanz-Morejón, and Nadia Mercader 6. Cardiac Resection Injury in Zebrafish Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi 7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish Fei Sun, Adam R. Shoffner, and Kenneth D. Poss 8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration Yijen L. Wu Part II: Ex Vivo and In Vivo Approaches 9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes Marina Leone and Felix B. Engel 10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes Lu Han, Jocelyn Mich-Basso, and Bernhard Kühn 11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra 12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells Jun Xu, Li Wang, Jiandong Liu, and Li Qian 13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs Abbigail Helfer and Nenad Bursac 14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells Ling Gao and Jianyi Zhang 15. Isolation[SL1] [DC2] and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts Honghai Liu, Kevin Bersell, and Bernhard Kühn 16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish Sierra Duca and Jingli Cao 17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, and Sean M. Wu Part III: Visualizing and Manipulating Heart Regeneration 18. In Vivo Clonal Analysis of Cardiomyocytes Ngoc B. Nguyen, G. Esteban Fernandez, Yichen Ding, Tzung Hsiai, and Reza Ardehali 19. High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry Frank Gyngard, Louise Trakimas, and Matthew L. Steinhauser 20. AAV Gene Transfer to the Heart Suya Wang, Yuxuan Guo, and William T. Pu 21. In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy Nishat Sultana, Mohammad Tofael Kabir Sharkar, Yoav Hadas, Elena Chepurko, and Lior Zangi 22. Generation and Manipulation of Exosomes Shiqi Hu, Li Qiao, and Ke Cheng 23. Epigenetic Assays in Purified Cardiomyocyte Nuclei Matthew C. Hill and James F. Martin 24. Genetic Lineage Tracing of Non-Cardiomyocytes in Mice Zhongming Chen and Jop H. van Berlo 25. Experimental Hypoxia as a Model for Cardiac Regeneration in Mice Yuji Nakada and Hesham A. Sadek [SL1] [DC2].
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2021
• Extent: 1 online resource (xiii, 346 pages), illustrations (some color)
• Subjects: 612.1/7045; Myocardium -- Regeneration -- Laboratory manuals; Regenerative medicine -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781071606681; 1071606689
• Related ISBNs: 9781071606674
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 7, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.