Gapmers : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Toshifumi Yokota, Rika Maruyama.
• Editors: Yokota, Toshifumi; Maruyama, Rika
• Contents: Part I: Basics and Introduction 1. Invention and Early History Kenji Rowel Q. Lim and Toshifumi Yokota 2. Development and Clinical Applications of Antisense Oligonucleotide Gapmers Leanna Chan and Toshifumi Yokota 3. Knocking Down Long Non-coding RNAs using Antisense Oligonucleotide Gapmers Rika Maruyama and Toshifumi Yokota 4. Development of Antisense Oligonucleotide Gapmers for the Treatment of Huntington's Disease Tejal Aslesh and Toshifumi Yokota 5. Development of Antisense Oligonucleotide Gapmers for the Treatment of Dyslipidemia and Lipodystrophy Tejal Aslesh and Toshifumi Yokota 6. Inotersen for the Treatment of Hereditary Transthyretin Amyloidosis Maria Mahfouz, Rika Maruyama, and Toshifumi Yokota 7. Degradation of Toxic RNA in Myotonic Dystrophy Using Gapmer Antisense Oligonucleotides Quynh Nguyen and Toshifumi Yokota Part II: Design of Gapmers and Strategy 8. DNA/RNA Heteroduplex Oligonucleotide for Highly Efficient Gene Silencing Rintaro Iwata Hara, Kotaro Yoshioka, and Takanori Yokota 9. Tips for Successful lncRNA Knockdown using Gapmers Kim A Lennox and Mark A Behlke 10. Calcium-Mediated In Vitro Transfection Technique of Oligonucleotides with Broad Chemical Modification Compatibility Fumito Wada, Shin-ichiro Hori, Satoshi Obika, and Tsuyoshi Yamamoto 11. Evaluating the Knockdown Activity of MALAT1 ENA Gapmers in vitro Shinzo Iwashita, Takao Shoji, and Makoto Koizumi 12. Albumin-binding Fatty Acid-modified Gapmer Antisense Oligonucleotides for Modulation of Pharmacokinetics Yunpeng Cai, Chenguang Lou, Jesper Wengel, and Kenneth A. Howard Part III: In Vitro/In Vivo Evaluation of Gapmers and Antisense Oligonucleotides 13. The use of Gapmers for in vivo Suppression of Hepatic mRNA Targets David S. Greenberg, Yonat Tzur, and Hermona Soreq 14. Development of LNA Gapmer Oligonucleotide-Based Therapy for ALS/ FTD Caused by the C9orf72 Repeat Expansion Chaitra Sathyaprakash, Raquel Manzano, Miguel A. Varela, Yasumasa Hashimoto, Matthew J. A. Wood, Kevin Talbot, and Yoshitsugu Aoki 15. Targeted Gene Silencing in Malignant Hematolymphoid Cells using GapmeR Atish Kizhakeyil, Mobashar Hussain Urf Turabe Fazil, and Navin Kumar Verma 16. Gapmer Antisense Oligonucleotides to Selectively Suppress the Mutant allele in COL6A Genes in Sominant Ullrich Congenital Muscular Dystrophy Sara Aguti, Elena Marrosu, Francesco Muntoni, and Haiyan Zhou Part IV: Detection of Gapmers 17. Detection of Locked Nucleic Acid Gapmers from Mouse Samples using ELISA Kenji Rowel Q. Lim, Quynh Nguyen, and Toshifumi Yokota.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource
• Subjects: 572.85; Triple-helix-forming oligonucleotides -- Laboratory manuals; Oligonucleotides, Antisense; Laboratory Manual; Electronic books
• Languages: English
• ISBNs: 9781071607718; 1071607715
• Related ISBNs: 1071607707; 9781071607701
• Notes: Note: Description based on online resource; title from digital title page (viewed on October 16, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.