Homologous recombination : methods and protocols /: methods and protocols. ([2021])

• Record Type: Book
• Further Information: Note: Edited by Andrés Aguilera, Aura Carreira.
• Editors: Aguilera, Andrés; Carreira, Aura
• Contents: Preface… Table of Contents… Contributing Authors… 1 Detection of DNA Double Strand Breaks by γ-H2AX Immunodetection Sonia I. Barroso and Andrés Aguilera 2 END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells Nancy Wong, Sam John, André Nussenzweig, and Andres Canela 3 Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting Erika Casari, Elisa Gobbini, Michela Clerici, and Maria Pia Longhese 4 Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe Zhenxin Yan, Sandeep Kumar, and Grzegorz Ira 5 Quantifying DNA End Resection in Human Cells Yi Zhou and Tanya T. Paull 6 Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure-Forming Repeats Anissia Ait Saada, Alex Costa, and Kirill S. Lobachev 7 Biochemical analysis of D-Loop Extension and DNA Strand Displacement Synthesis Youngho Kwon and Patrick Sung 8 DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing Sudipta Lahiri and Ryan B. Jensen 9 Monitoring Homologous Recombination Activity in Human Cells Domagoj Vugic, Åsa Ehlén, and Aura Carreira 10 Inter-Homolog Homologous Recombination in Mouse Embryonic Stem Cells Fabio Vanoli, Rohit Prakash, Travis White, and Maria Jasin 11 Branch Migration Activity of Rad54 Protein Olga M. Mazina and Alexander V. Mazin 12 Holliday Junction Resolution Raquel Carreira, F. Javier Aguado, Tomas Lama-Diaz, and Miguel G. Blanco 13 Identification and Analysis of Different Types of UFBs Simon Gemble and Mounira Amor-Guéret 14 Intrachromosomal Recombination in Yeast Anastasiya Epshtein, Lorraine S. Symington, and Hannah L. Klein 15 Genome-Wide Analysis of Mitotic Recombination in Budding Yeast Lydia R. Heasley, Nadia M. V. Sampaio, and Juan Lucas Argüeso 16 Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis Miyuki Yamaguchi and James E. Haber 17 DNA Double-Strand Break Induced Gene Amplification in Yeast Tomas Strucko, Michael Lisby, and Uffe Hasbro Mortensen 18 Measuring Chromosome Pairing during Homologous Recombination in Yeast Fraulin Joseph, So Jung Lee, Eric Bryant, and Rodney Rothstein 19 Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes Yan Yun, Masaru Ito, Sumit Sandhu, and Neil Hunter 20 Detection of DSBs in C. elegans Meiosis Tatiana García-Muse 21 Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae Aurore Sanchez and Valérie Borde 22 Investigation of Break-Induced Replication in Yeast Beth Osia, Rajula Elango, Juraj Kramara, Steven A. Roberts, and Anna Malkova 23 Measurement of Homologous Recombination at Stalled Mammalian Replication Forks Nicholas A. Willis and Ralph Scully 24 Super Resolution Imaging of Homologous Recombination Repair at Collapsed Replication Forks Donna R. Whelan and Eli Rothenberg 25 The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bi-Dimensional Gel Electrophoresis Karol Kramarz, Anissia Ait Saada and Sarah A.E. Lambert 26 The Sister Chromatid Exchange Assay in Human Cells Emanuela Tumini and Andrés Aguilera 27 Analysis of Recombination at Yeast Telomeres Marie-Noelle Simon, Dmitri Churikov and Vincent Géli 28 Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae Mariko Sasaki and Takehiko Kobayashi 29 Analyzing Homologous Recombination at a Genome Wide Level Coline Arnould, Vincent Rocher, and Gaëlle Legube 30 CRISPR/Cas9 Induced Breaks in Heterochromatin, Visualized by Immunofluorescence Mitrentsi Ioanna and Evi Soutoglou 31 In Vivo Binding of Recombination Proteins of Non-DSB DNA Lesions to Replication Forks Román González-Prieto, María J. Cabello-Lobato and Félix Prado 32 Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells Colby See, Deepak Arya, Emily Lin, and Irene Chiolo 33 In Vitro Characterization of Sumoylation of HR Proteins Veronika Altmannova and Lumir Krejci 34 High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products Dionna Gamble, Yee Fang Hum, and Sue Jinks-Robertson 35 Fluorescence Microscopy for Analysis of Relocalization of Structure-Specific Endonucleases Carl P. Lehmann, Irene Saugar, and José Antonio Tercero 36 Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-Invasion-Induced Rearrangements in S. cerevisiae Aurèle Piazza, Pallavi Rajput, and Wolf-Dietrich Heyer.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2021
• Extent: 1 online resource (xv, 557 pages), illustrations (some color)
• Subjects: 572.8/77; Genetic recombination -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781071606445; 1071606441
• Related ISBNs: 9781071606438
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 7, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.