Coronaviruses : Methods and Protocols /: Methods and Protocols. (2020)

• Record Type: Book
• Further Information: Note: Helena J. Maier, Erica Bickerton.
• Editors: Maier, Helena J; Bickerton, Erica
• Contents: 1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis Yuhang Wang, Matthew Grunewald, and Stanley Perlman Part I: Coronavirus Detection, Discovery, and Evolution 2. Discovery of Novel Coronaviruses in Rodents Theocharis Tsoleridis and Jonathan K. Ball 3. Detection and Discovery of Coronaviruses in Wild Bird Populations Chantal J. Snoeck and Siamak Zohari 4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) Shuetsu Fukushi 5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing Graham L. Freimanis and Michael S. Oade Part II: Propagation and Titration of Coronaviruses 6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif 7. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation Elena Lokhman, Srijana Rai, and Billy Matthews 8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation Giulia Dowgier and Erica Bickerton 9. Isolation and Propagation of Coronaviruses in Embryonated Eggs James S. Guy 10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures Mitra Gultom, Laura Laloli, and Ronald Dijkman 11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo Giulia Dowgier and Helena J. Maier Part III: Manipulating the Genomes of Coronaviruses 12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses Sarah Keep, Paul Britton, and Erica Bickerton 13. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel Part IV: Studying Virus-Host Interactions 14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions Philip V'kovski, Silvio Steiner, and Volker Thiel 15. Using Yeast to Identify Coronavirus-Host Protein Interactions Stuart Weston and Matthew Frieman 16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response Christopher M. Coleman 17. Ribopuromycylation in Coronavirus-Infected Cells Matthew J. Brownsword, Helena J. Maier, and Nicolas Locker Part V: Imaging Coronavirus Infections 18. Visualizing Coronavirus Entry into Cells Aleksandra Milewska, Katarzyna Owczarek, Artur Szczepanski, and Krzysztof Pyrc 19. Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope Nicole Doyle and Philippa C. Hawes.
• Edition: Second edition
• Publisher Details: New York : Humana
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource (278 pages)
• Subjects: Medicine; Virology; Medical -- Microbiology; Medical microbiology & virology
• Languages: English
• ISBNs: 9781071609002
• Related ISBNs: 9781071608999
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.