DNA vaccines : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Ângela Sousa.
• Editors: Sousa, Ângela (Ângela Maria Almeida de)
• Contents: Part I: Vector design For vaccination 1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application Yanlong Jiang, Guilian Yang, and Chunfeng Wang 2. Vaccination with Messenger RNA – a Promising Alternative to DNA Vaccination István Tombácz, Drew Weissman, and Norbert Pardi 3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency Kenneth Lundstrom Part II: DNA Vaccine Adjuvants and Immunostimulatory Response 4. CpG Oligonucleotides as Vaccine Adjuvants Neslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman 5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation Ailar Sabbaghi and Amir Ghaemi 6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model Xintao Hu, Barbara K. Felber, and Antonio Valentin Part III Biotechnological Processes to Obtain DNA Vaccines 7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design Luís A. Passarinha 8. Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres 9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths Urh Černigoj and Aleš Štrancar 10. Purification of Plasmid DNA by Multimodal Chromatography A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo 11. Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment Ana M. Almeida, Dalinda Eusébio, João A. Queiroz, Fani Sousa, and Ângela Sousa Part IV: DNA Vaccine Delivery 12. Tumor-Specific CD8+ T Cell Responses Induced by DNA Vaccination Pablo Cáceres-Morgado and Alvaro Lladser 13. Therapeutic DNA Vaccine Against HPV16-Associated Cancer Meihua Yu and Janin Chandra 14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications Lucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli 15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field Diana Costa, Ângela Sousa, Rúben Faria, Ana Raquel Neves, and João A. Queiroz 16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols Nina D. Coelho-Rocha, Fernanda A. L. Barroso, Laísa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-Agresti Part V: DNA Vaccine Transfer to Clinical Trials 17. Ethics of DNA Vaccines Transfer for Clinical Research Ana Cristina Ramalhinho and Miguel Castelo-Branco 18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trial Ana Cristina Ramalhinho and Miguel Castelo-Branco.
• Publisher Details: New York : Humana
• Publication Date: 2020
• Copyright Date: 2021
• Extent: 1 online resource (335 pages)
• Subjects: 615.3/72; DNA vaccines -- Laboratory manuals; DNA vaccines -- Research -- Methodology; Vaccines, DNA; DNA vaccines; Laboratory manuals; Laboratory Manual
• Languages: English
• ISBNs: 9781071608722
• Related ISBNs: 9781071608715
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.