Engineering of microbial biosynthetic pathways. (2020)

• Record Type: Book
• Further Information: Note: Vijai Singh, Ajay Kumar Singh, Poonam Bhargava, Madhvi Joshi, Chaitanya G. Joshi, editors.
• Other Names: Singh, Vijai; Singh, Ajay Kumar; Bhargava, Poonam; Joshi, Madhvi; Joshi, Chaitanya G
• Contents: Intro -- Foreword -- Preface -- Acknowledgments -- Contents -- About the Editors -- 1: An Introduction to Design of Microbial Strain using Synthetic Biology Toolboxes for Production of Biomolecules -- 1.1 Introduction -- 1.2 Desired Characteristics of Strain Used for Production of Biomolecules -- 1.3 Methods Used for Strain Improvement -- 1.4 Protein Engineering -- 1.5 Directed Evolution -- 1.6 Design and Construction of Biosynthetic Pathways -- 1.7 Synthetic Biology Toolbox in Metabolic Engineering -- 1.8 Adaptive Laboratory Evolution -- 1.9 Conclusion and Future Remarks -- References 2: Microbial Strain Engineering -- 2.1 Introduction -- 2.1.1 Need and Significance for Strain Improvement -- 2.2 Mutagenesis -- 2.2.1 Physical and Chemical Mutagenesis -- 2.2.2 Mutation Signature -- 2.3 Engineering Physiology of Microbes -- 2.3.1 Desired Physiological Characteristics -- 2.3.2 Candidate Screening -- 2.3.3 Selection of Host Strain -- 2.3.4 Engineering into the Host Strain -- 2.4 Metabolic Engineering -- 2.4.1 Methodologies and Tools for Metabolic Engineering -- 2.4.2 Engineering of Biosynthetic Pathways -- 2.4.3 Central Metabolism Engineering -- 2.4.4 Transport Engineering 2.4.5 Engineering the Whole Cell -- 2.5 Genetic Engineering -- 2.5.1 Screening -- 2.5.2 Gene Expression -- 2.5.3 Enzyme (Over)Production and Posttranscriptional Control -- 2.6 Omics for Strain Engineering -- 2.6.1 Genome Analysis -- 2.6.2 Transcriptome Analysis -- 2.6.3 Proteome Analysis -- 2.6.4 Fluxome and Metabolome Analysis -- 2.6.4.1 Metabolome Analysis -- 2.6.4.2 Fluxome Analysis -- 2.6.5 Combined Omics Approach -- 2.7 Conclusion -- References -- 3: Techniques for Detection and Extraction of Metabolites -- 3.1 Introduction -- 3.2 Extraction of Metabolites -- 3.2.1 Quenching Methods 3.2.1.1 Perchloric Acid -- 3.2.1.2 Liquid Nitrogen Method -- 3.2.1.3 Methanol Method -- 3.2.2 Extraction of Extracellular Metabolites -- 3.2.3 Extraction of Intracellular Metabolites -- 3.2.3.1 Non-mechanical Lysis -- Boiling Ethanol -- Cold Methanol -- Buffered Methanol-Chloroform-Water -- Hot Water -- 3.2.3.2 Mechanical Lysis of Cell -- Supercritical Fluid Extraction -- Pressurized Liquid Extraction -- 3.3 Detection of Metabolites -- 3.3.1 Mass-Spectrometry (MS) -- 3.3.2 Gas Chromatography-MS -- 3.3.3 Capillary Electrophoresis-MS (CE-MS) -- 3.3.4 Liquid Chromatography-MS (LC-MS) 3.3.5 Nuclear Magnetic Resonance -- 3.4 Conclusion -- References -- 4: Genetically Encoded Biosensors and Their Applications in the Development of Microbial Cell Factories -- 4.1 The Classification of Genetically Encoded Biosensors -- 4.1.1 Protein-Based Biosensors -- 4.1.1.1 The Functional Mechanism of Protein-Based Biosensors -- 4.1.1.2 Designing and Tuning Protein-Based Biosensors -- Tuning at aTF Level -- Tuning at Promoter Level -- 4.1.2 RNA-Based Biosensors -- 4.1.2.1 The Functional Mechanism of RNA-Based Biosensors -- 4.1.2.2 Designing and Tuning RNA-Based Biosensors
• Publisher Details: Singapore : Springer
• Publication Date: 2020
• Extent: 1 online resource (326 p.)
• Subjects: 660.6/2; Microbial biotechnology; Biotechnological microorganisms; Electronic books; Electronic books
• Languages: English
• ISBNs: 9789811526046; 9811526044
• Related ISBNs: 9811526036; 9789811526039
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.