Plant endosomes : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Marisa S. Otegui.
• Editors: Otegui, Marisa S
• Contents: 1. Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis Akira Yoshinari and Junpei Takano 2. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles Mengqian Luo, Ying Zhu, Zhiqi Liu, and Liwen Jiang 3. Analysis of Endoplasmic Reticulum-Endosomes Association Using Live-Cell Imaging In Plant Cells Giovanni Stefano and Federica Brandizzi 4. Degradation of Abscisic Acid Receptors through the Endosomal Pathway Borja Belda-Palazón and Pedro L. Rodriguez 5. Biochemical and Imaging Analysis of ALIX Function in the Endosomal Trafficking of Arabidopsis Protein Cargoes Marta García-León and Vicente Rubio 6. Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network Pengfei Wang and Byung-Ho Kang 7. Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy Kirk Czymmek, Abhilash Sawant, Kaija Goodman, Janice Pennington, Pal Pedersen, Mrinalini Hoon, and Marisa S. Otegui 8. Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking Wenjin Shen, Juan Wei, and Caiji Gao 9. Assessing Extrinsic Membrane Protein Dependency to PI4P using a Plasma Membrane to Endosome Re-localisation Transient Assay in Nicotiana benthamiana Mehdi Doumane and Marie-Cécile Caillaud 10. Subcellular Localization of PI3P in Arabidopsis Han Nim Lee, Hyera Jung, and Taijoon Chung 11. Immuno-purification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis Yoko Ito, Magali Grison, Nicolas Esnay, Laetitia Fouillen, and Yohann Boutté 12. Cell-free Protein Translation System For Expression Of Lipid-binding Proteins Tagged with Small Epitopes and their use in Protein-lipid Overlay Assays Julio Paez-Valencia and Marisa S. Otegui 13. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants Guangxi Ren, Michel Ruiz Rosquete, Angelo G. Peralta, Sivakumar Pattathil, Michael G. Hahn, Thomas Wilkop, and Georgia Drakakaki 14. Detection of Phosphorylation on Immunoprecipitates from Total Protein Extracts of Arabidopsis thaliana Seedlings Karin Vogel and Erika Isono 15. Purification and Interaction Analysis of a Plant-specific RAB5 Effector by in vitro pull-down Assay Emi Ito, Seung-won Choi, and Takashi Ueda.
• Edition: Second edition
• Publisher Details: New York : Humana
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource (200 pages)
• Subjects: 571.6/5; Endosomes -- Laboratory manuals; Plant organelles -- Laboratory manuals; Endosomes; Plant Proteins; Endosomes; Plant organelles; Laboratory manuals
• Languages: English
• ISBNs: 9781071607671
• Related ISBNs: 9781071607664
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.