Frontiers in protein structure, function, and dynamics. (2020)
- Record Type:
- Book
- Title:
- Frontiers in protein structure, function, and dynamics. (2020)
- Main Title:
- Frontiers in protein structure, function, and dynamics
- Further Information:
- Note: Dev Bukhsh Singh, Timir Tripathi, editors.
- Other Names:
- Singh, Dev Bukhsh
Tripathi, Timir - Contents:
- Intro -- Preface -- Contents -- About the Editors -- 1: Protein Purification, Estimation, Storage, and Effect on Structure-Function-Dynamics -- 1.1 Introduction -- 1.2 Protein Purification -- 1.2.1 Protein Precipitation/Salting Out -- 1.2.2 Buffer Exchange/Dialysis -- 1.2.3 Immuno-Affinity Chromatography -- 1.2.4 Affinity Chromatography -- 1.2.5 Ion-Exchange Chromatography -- 1.2.6 Size-Exclusion or Gel Filtration Chromatography (SEC/GFC) -- 1.2.7 High-Pressure/Performance Liquid Chromatography (HPLC) -- 1.2.8 Hydrophobic Interaction Chromatography -- 1.3 Resolving and Display of Protein 1.6.3 Cycles of Freeze-Thaw -- 1.6.4 Organic Solvents and pH -- 1.6.5 Proteases/Peptidases -- 1.6.6 Protein Concentration -- 1.6.7 Reducing Agents -- 1.6.8 Salt Conditions and Urea -- 1.6.9 Temperature -- 1.7 Factors Affecting the Stability of Proteins -- 1.8 Effect on the Dynamics of Protein -- 1.9 Conclusions -- References -- 2: Experimental and Computational Methods to Determine Protein Structure and Stability -- 2.1 Protein Purification Techniques -- 2.1.1 General Aspects of Protein Purification -- 2.1.2 Stabilizing Proteins -- 2.1.3 Quantification of Proteins 2.1.4 Purification of Proteins Based on Solubility -- 2.1.5 Purification of Proteins Based on Size -- 2.1.5.1 Ultrafiltration -- 2.1.5.2 Gel Filtration or Molecular Sieve or Size Exclusion Chromatography -- 2.1.5.3 SDS-PAGE -- 2.1.6 Purification of Proteins Based on Charge -- 2.1.6.1 Ion-Exchange Chromatography -- 2.1.6.2Intro -- Preface -- Contents -- About the Editors -- 1: Protein Purification, Estimation, Storage, and Effect on Structure-Function-Dynamics -- 1.1 Introduction -- 1.2 Protein Purification -- 1.2.1 Protein Precipitation/Salting Out -- 1.2.2 Buffer Exchange/Dialysis -- 1.2.3 Immuno-Affinity Chromatography -- 1.2.4 Affinity Chromatography -- 1.2.5 Ion-Exchange Chromatography -- 1.2.6 Size-Exclusion or Gel Filtration Chromatography (SEC/GFC) -- 1.2.7 High-Pressure/Performance Liquid Chromatography (HPLC) -- 1.2.8 Hydrophobic Interaction Chromatography -- 1.3 Resolving and Display of Protein 1.6.3 Cycles of Freeze-Thaw -- 1.6.4 Organic Solvents and pH -- 1.6.5 Proteases/Peptidases -- 1.6.6 Protein Concentration -- 1.6.7 Reducing Agents -- 1.6.8 Salt Conditions and Urea -- 1.6.9 Temperature -- 1.7 Factors Affecting the Stability of Proteins -- 1.8 Effect on the Dynamics of Protein -- 1.9 Conclusions -- References -- 2: Experimental and Computational Methods to Determine Protein Structure and Stability -- 2.1 Protein Purification Techniques -- 2.1.1 General Aspects of Protein Purification -- 2.1.2 Stabilizing Proteins -- 2.1.3 Quantification of Proteins 2.1.4 Purification of Proteins Based on Solubility -- 2.1.5 Purification of Proteins Based on Size -- 2.1.5.1 Ultrafiltration -- 2.1.5.2 Gel Filtration or Molecular Sieve or Size Exclusion Chromatography -- 2.1.5.3 SDS-PAGE -- 2.1.6 Purification of Proteins Based on Charge -- 2.1.6.1 Ion-Exchange Chromatography -- 2.1.6.2 Isoelectric Focusing -- 2.1.6.3 Polyacrylamide Gel Electrophoresis -- 2.1.7 Purification of Proteins Based on Polarity (Hydrophobic Interaction Chromatography) -- 2.1.8 Purification of Proteins Based on theBinding Property (Affinity Chromatography) 2.2 Biophysical and Biochemical Characterization of Protein -- 2.2.1 Biophysical Characterization Methods -- 2.2.2 Dynamic Light Scattering (DLS) -- 2.2.2.1 Applications of DLS -- 2.2.3 Circular Dichroism (CD) Spectroscopy -- 2.2.3.1 Applications of CD -- 2.2.3.2 Advantages of CD -- 2.2.4 Differential Scanning Calorimetry (DSC) -- 2.2.4.1 Applications of DSC -- 2.2.5 Fourier-Transform Infrared (FTIR) Spectroscopy -- 2.2.5.1 Advantages of FTIR -- 2.2.6 Isothermal Titration Calorimetry (ITC) -- 2.2.6.1 Applications of ITC -- 2.2.7 Biochemical Characterization -- 2.2.8 Oxidation -- 2.2.9 Deamidation … (more)
- Publisher Details:
- Singapore : Springer
- Publication Date:
- 2020
- Extent:
- 1 online resource
- Subjects:
- 572/.6
Proteins
Proteins -- Structure
Protein Conformation
Protein Folding
Proteins
Enzymes
Electronic books
Electronic books - Languages:
- English
- ISBNs:
- 9789811555305
9811555303 - Related ISBNs:
- 981155529X
9789811555299 - Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
- Access Usage:
- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.515408
- Ingest File:
- 03_098.xml